Enhancing nisin yield by engineering a small noncodding RNA anti41 and inhibiting the expression of glnR in Lactococcus lactis F44
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To engineer a small nonconding RNA anti41 to enhance nisin yield by inhibiting the expression of glnR in Lactococcus lactis F44.
We constructed a screening library to determine appropriate artificial sRNAs and obtained a sRNA anti41 that can produce approximately three fold of the inhibitory effect on GlnR. Moreover, the transcription levels of the direct inhibitory targets of GlnR (glnP, glnQ, amtB, and glnK) were dramatically upregulated in the anti41 overexpression strain (F44-anti41), thereby confirming the inhibitory effect of anti41 on GlnR. In addition, anti41 overexpression improved the survival rate of cells by approximately three fold under acid stress, promoted cell growth, and increased nisin yield by 29.83%.
We were able to provide a novel strategy for the construction of robust high-producing industrial strains.
KeywordsLactococcus lactis Artificial sRNA GlnR Acid resistance Nisin yield
This study was supported by the National Key R&D Program of China (2017YFD0201405), the National Natural Science Foundation of China (31270142, 31770076, and 31570089), and the Funds for Creative Research Groups of China (21621004). Dr. Jianjun Qiao was supported by The New Century Outstanding Talent Support Program, Education Ministry of China.
Table S1—Oligonucleotides used in this work.
Compliance with ethical standards
Conflict of interest
The authors declare no competing interests.