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Transcriptional regulator XYR1 activates the expression of cellobiose synthase to promote the production of cellulase from glucose

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Abstract

Objective

To investigate the transcriptional regulation of cellobiose synthase (CBS) in Rhizopus stolonifer.

Results

Transcription factor XYR1 was identified as responsible for the activation of cbs. In comparison with wild-type R. stolonifer, the deletion of XYR1 resulted in transcriptional down-regulation of cbs by approximately 40%, while XYR1 over-expression increased cbs transcription up to 175%. The highest FPA activity (1.8 IU/ml) was obtained in the XYR1-overexpressing strain OExyr1 cultivated in a 2% (m/V) glucose media, corresponding to a 96% increase compared with that of the parent strain (0.92 IU/ml). Moreover, cellulase synthesis was inhibited after cbs-inactivation mutation in OExyr1.

Conclusion

XYR1 directly activates the transcription of cbs to promote cellulase production in R. stolonifer utilizing glucose as a substrate.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31270135).

Supporting information

Supplementary Table 1—The sequences of primers for TAIL-PCR.

Supplementary Table 2—The sequences of primers for overlapping-PCR.

Supplementary Table 3—The sequences of primers for RT-qPCR.

Author information

Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Yingying Zhang, Bin Tang & Guocheng Du

  2. College of Biochemical Engineering, Anhui Polytechnic University, Wuhu, 241000, People’s Republic of China

    Bin Tang

Authors
  1. Yingying Zhang
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  2. Bin Tang
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  3. Guocheng Du
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Corresponding author

Correspondence to Bin Tang.

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Zhang, Y., Tang, B. & Du, G. Transcriptional regulator XYR1 activates the expression of cellobiose synthase to promote the production of cellulase from glucose. Biotechnol Lett 40, 973–979 (2018). https://doi.org/10.1007/s10529-018-2549-9

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  • DOI: https://doi.org/10.1007/s10529-018-2549-9

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