A versatile Trichoderma reesei expression system for the production of heterologous proteins
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To develop a versatile Trichoderma reesei (teleomorph Hypocrea jecorina) expression system for the high-purity production of heterologous proteins.
The versatile T. reesei expression system is based on xyn1 and xyn2 promoters, A824V transition in XYRI, and a bicomponent carbon source strategy. Red fluorescent protein gene rfp and alkaline endoglucanase EGV gene egv3 from Humicola insolens were used as reporter genes to test our versatile expression system
The versatile T. reesei expression system can be applied to produce heterologous proteins with high purity and high yield.
KeywordsHeterologous proteins Promoter Trichoderma reesei Versatile expression system xyn1 xyn2
This research was supported by the National Natural Science Foundation of China [No. C010302-31500066] and the Fundamental Research Funds for the Central Universities [No. 222201714053].
Supplementary Table 1—Primers for pXMG, pX1rfp, pX2rfp, pX1egv3 and pX2egv3 constructed in this study.
Supplementary Figure 1—Schematic for identification of gene size and single-copy DNA integration in the genome of the transformants.
Supplementary Figure 2—SDS-PAGE analysis of extracellular proteins secreted by T. reesei RUT C30 and TXMG in different cultivation strategies.
Compliance with ethical standards
Conflict of interest
The author(s) declare that they have no competing interests.