A novel strategy for production of ethanol and recovery of xylose from simulated corncob hydrolysate
- 223 Downloads
To develop a xylose-nonutilizing Escherichia coli strain for ethanol production and xylose recovery.
Xylose-nonutilizing E. coli CICIM B0013-2012 was successfully constructed from E. coli B0013-1030 (pta-ack, ldhA, pflB, xylH) by deletion of frdA, xylA and xylE. It exhibited robust growth on plates containing glucose, arabinose or galactose, but failed to grow on xylose. The ethanol synthesis pathway was then introduced into B0013-2012 to create an ethanologenic strain B0013-2012PA. In shaking flask fermentation, B0013-2012PA fermented glucose to ethanol with the yield of 48.4 g/100 g sugar while xylose remained in the broth. In a 7-l bioreactor, B0013-2012PA fermented glucose, galactose and arabinose in the simulated corncob hydrolysate to 53.4 g/l ethanol with the yield of 48.9 g/100 g sugars and left 69.6 g/l xylose in the broth, representing 98.6% of the total xylose in the simulated corncob hydrolysate.
By using newly constructed strain B0013-2012PA, we successfully developed an efficient bioprocess for ethanol production and xylose recovery from the simulated corncob hydrolysate.
KeywordsEscherichia coli Ethanol Simulated corncob hydrolysate Xylose recovery
This research was supported financially by The Raising Program of Innovation Team for Tianjin Universities (TD12-5002).
Supplementary Table 1—Primers used in this study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Andersen RL, Jensen KM, Mikkelsen MJ (2015) Continuous ethanol fermentation of pretreated lignocellulosic biomasses, waste biomasses, molasses and syrup using the anaerobic, thermophilic bacterium Thermoanaerobacter italicus Pentocrobe 411. PLoS ONE 10:e0136060CrossRefPubMedPubMedCentralGoogle Scholar
- Cao JL, Zhou L, Zhang L, Wang ZX, Shi GY (2010) Construction and fermentation of succinate-producing recombinant Escherichia coli. Chin J Appl Environ Biol 16:851–857Google Scholar
- Garcia Sanchez R, Karhumaa K, Fonseca C, Sànchez Nogué V, Almeida JR, Larsson CU, Bengtsson O, Bettiga M, Hahn-Hägerdal B, Gorwa-Grauslund MF (2010) Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering. Biotechnol Biofuels 3:13CrossRefPubMedPubMedCentralGoogle Scholar
- Kogje A, Ghosalkar A (2016) Xylitol production by Saccharomyces cerevisiae overexpressing different xylose reductases using non-detoxified hemicellulosic hydrolysate of corncob. 3. Biotech 6:127Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Sun JF, Xu M, Zhang F, Wang ZX (2004) Novel recombinant Escherichia coli producing ethanol from glucose and xylose. Acta Microbiol Sin 44:600–604Google Scholar
- Sun JF, Tian KM, Shen W, Chen XZ, Wang ZX (2017) Genetic nature of xylose metabolism, differences between Escherichia coli strains. Chin J Food Ferment Ind 43:68–73Google Scholar
- Treebupachatsakul T, Shioya K, Nakazawa H, Kawaguchi T, Morikawa Y, Shida Y, Ogasawara W, Okada H (2015) Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass. J Biosci Bioeng 120:657–665CrossRefPubMedGoogle Scholar