Skip to main content

Advertisement

SpringerLink
Log in

A novel strategy for production of ethanol and recovery of xylose from simulated corncob hydrolysate

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objectives

To develop a xylose-nonutilizing Escherichia coli strain for ethanol production and xylose recovery.

Results

Xylose-nonutilizing E. coli CICIM B0013-2012 was successfully constructed from E. coli B0013-1030 (pta-ack, ldhA, pflB, xylH) by deletion of frdA, xylA and xylE. It exhibited robust growth on plates containing glucose, arabinose or galactose, but failed to grow on xylose. The ethanol synthesis pathway was then introduced into B0013-2012 to create an ethanologenic strain B0013-2012PA. In shaking flask fermentation, B0013-2012PA fermented glucose to ethanol with the yield of 48.4 g/100 g sugar while xylose remained in the broth. In a 7-l bioreactor, B0013-2012PA fermented glucose, galactose and arabinose in the simulated corncob hydrolysate to 53.4 g/l ethanol with the yield of 48.9 g/100 g sugars and left 69.6 g/l xylose in the broth, representing 98.6% of the total xylose in the simulated corncob hydrolysate.

Conclusions

By using newly constructed strain B0013-2012PA, we successfully developed an efficient bioprocess for ethanol production and xylose recovery from the simulated corncob hydrolysate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Anasontzis GE, Christakopoulos P (2014) Challenges in ethanol production with Fusarium oxysporum through consolidated bioprocessing. Bioengineered 5:393–395

    Article  PubMed  PubMed Central  Google Scholar 

  • Andersen RL, Jensen KM, Mikkelsen MJ (2015) Continuous ethanol fermentation of pretreated lignocellulosic biomasses, waste biomasses, molasses and syrup using the anaerobic, thermophilic bacterium Thermoanaerobacter italicus Pentocrobe 411. PLoS ONE 10:e0136060

    Article  PubMed  PubMed Central  Google Scholar 

  • Banerjee S, Mudliar S, Sen R, Giri B, Satpute D, Chakrabarti T, Pandey R (2010) Commercializing lignocellulosic bioethanol: technology bottlenecks and possible remedies. Biofuel Bioprod Bior 4:77–93

    Article  CAS  Google Scholar 

  • Bátori V, Ferreira JA, Taherzadeh MJ, Lennartsson PR (2015) Ethanol and protein from ethanol plant by-products using edible fungi Neurospora intermedia and Aspergillus oryzae. Biomed Res Int 2015:176371

    Article  PubMed  PubMed Central  Google Scholar 

  • Cao JL, Zhou L, Zhang L, Wang ZX, Shi GY (2010) Construction and fermentation of succinate-producing recombinant Escherichia coli. Chin J Appl Environ Biol 16:851–857

    CAS  Google Scholar 

  • Cirino PC, Chin JW, Ingram LO (2006) Engineering Escherichia coli for xylitol production from glucose-xylose mixtures. Biotechnol Bioeng 95:1167–1176

    Article  CAS  PubMed  Google Scholar 

  • Garcia Sanchez R, Karhumaa K, Fonseca C, Sànchez Nogué V, Almeida JR, Larsson CU, Bengtsson O, Bettiga M, Hahn-Hägerdal B, Gorwa-Grauslund MF (2010) Improved xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain using evolutionary engineering. Biotechnol Biofuels 3:13

    Article  PubMed  PubMed Central  Google Scholar 

  • Hasona A, Kim Y, Healy FG, Ingram LO, Shanmugam KT (2004) Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xylose. J Bacteriol 186:7593–7600

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ho NW, Chen Z, Brainard AP (1998) Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Appl Environ Microbiol 64:1852–1859

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ingram LO, Conway T, Clark DP, Sewell GW, Preston JF (1987) Genetic engineering of ethanol production in Escherichia coli. Appl Environ Microbiol 53:2420–2425

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jeffries TW (2006) Engineering yeasts for xylose metabolism. Curr Opin Biotechnol 17:320–326

    Article  CAS  PubMed  Google Scholar 

  • Khankal R, Chin JW, Cirino PC (2008) Role of xylose transporters in xylitol production from engineered Escherichia coli. J Biotechnol 134:246–252

    Article  CAS  PubMed  Google Scholar 

  • Kogje A, Ghosalkar A (2016) Xylitol production by Saccharomyces cerevisiae overexpressing different xylose reductases using non-detoxified hemicellulosic hydrolysate of corncob. 3. Biotech 6:127

    Google Scholar 

  • Latif F, Rajoka MI (2001) Production of ethanol and xylitol from corn cobs by yeasts. Bioresour Technol 77:57–63

    Article  CAS  PubMed  Google Scholar 

  • Ohta K, Beall DS, Mejia JP, Shanmugam KT, Ingram LO (1991) Metabolic engineering of Klebsiella oxytoca M5A1 for ethanol production from xylose and glucose. Appl Environ Microbiol 57:2810–2815

    CAS  PubMed  PubMed Central  Google Scholar 

  • Puseenam A, Tanapongpipat S, Roongsawang N (2015) Co-expression of endoxylanase and endoglucanase in Scheffersomyces stipitis and its application in ethanol production. Appl Biochem Biotechnol 177:1690–1700

    Article  CAS  PubMed  Google Scholar 

  • Rubin EM (2008) Genomics of cellulosic biofuels. Nature 454:841–845

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Song S, Park C (1997) Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator. J Bacteriol 179:7025–7032

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sun JF, Xu M, Zhang F, Wang ZX (2004) Novel recombinant Escherichia coli producing ethanol from glucose and xylose. Acta Microbiol Sin 44:600–604

    CAS  Google Scholar 

  • Sun JF, Tian KM, Shen W, Chen XZ, Wang ZX (2017) Genetic nature of xylose metabolism, differences between Escherichia coli strains. Chin J Food Ferment Ind 43:68–73

    Google Scholar 

  • Treebupachatsakul T, Shioya K, Nakazawa H, Kawaguchi T, Morikawa Y, Shida Y, Ogasawara W, Okada H (2015) Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass. J Biosci Bioeng 120:657–665

    Article  CAS  PubMed  Google Scholar 

  • Ur-Rehman S, Mushtaq Z, Zahoor T, Jamil A, Murtaza MA (2015) Xylitol: a review on bioproduction, application, health benefits, and related safety issues. Crit Rev Food Sci Nutr 55:1514–1528

    Article  CAS  PubMed  Google Scholar 

  • van Maris AJ, Winkler AA, Kuyper M, de Laat WT, van Dijken JP, Pronk JT (2007) Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component. Adv Biochem Eng Biotechnol 108:179–204

    PubMed  Google Scholar 

  • Yoon SH, Mukerjea R, Robyt JF (2003) Specificity of yeast (Saccharomyces cerevisiae) in removing carbohydrates by fermentation. Carbohydr Res 338:1127–1132

    Article  CAS  PubMed  Google Scholar 

  • Zhang M, Eddy C, Deanda K, Finkelstein M, Picataggio S (1995) Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267:240–243

    Article  CAS  PubMed  Google Scholar 

  • Zhou L, Zuo ZR, Chen XZ, Niu DD, Tian KM, Prior BA, Shen W, Shi GY, Singh S, Wang ZX (2011) Evaluation of genetic manipulation strategies on D-lactate production by Escherichia coli. Curr Microbiol 62:981–989

    Article  CAS  PubMed  Google Scholar 

  • Zhou L, Niu DD, Tian KM, Chen XZ, Prior BA, Shen W, Shi GY, Singh S, Wang ZX (2012) Genetically switched D-lactate production in Escherichia coli. Metab Eng 14:560–568

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported financially by The Raising Program of Innovation Team for Tianjin Universities (TD12-5002).

Supporting information

Supplementary Table 1—Primers used in this study.

Author information

Authors and Affiliations

  1. School of Biotechnology, Center for Bioresource and Bioenergy, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China

    Jinfeng Sun

  2. Department of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China

    Jie Wang, Kangming Tian, Zixing Dong, Xiaoguang Liu & Zhengxiang Wang

  3. School of Life Science and Food Engineering, Huaiyin Institute of Technology, 1st East Meicheng Road, Huaian, 223003, China

    Jinfeng Sun

  4. Department of Biotechnology and Food Technology, Durban University of Technology, Durban, 4002, South Africa

    Kugenthiren Permaul & Suren Singh

  5. Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    Bernard A. Prior

Authors
  1. Jinfeng Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jie Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kangming Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zixing Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaoguang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kugenthiren Permaul
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Suren Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bernard A. Prior
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zhengxiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhengxiang Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, J., Wang, J., Tian, K. et al. A novel strategy for production of ethanol and recovery of xylose from simulated corncob hydrolysate. Biotechnol Lett 40, 781–788 (2018). https://doi.org/10.1007/s10529-018-2537-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-018-2537-0

Keywords

Search

Navigation