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Biotechnology Letters

, Volume 40, Issue 4, pp 641–648 | Cite as

PKM2 knockdown influences SREBP activation and lipid synthesis in bovine mammary-gland epithelial MAC-T cells

  • Bing-Qian Su
  • Ying-Qian Han
  • Shuang-Shuang Fan
  • Sheng-Li Ming
  • Bo Wan
  • Wei-Fei Lu
  • Bei-Bei Chu
  • Guo-Yu Yang
  • Jiang Wang
Original Research Paper

Abstract

Objective

The purpose of the article is to evaluate the changes in lipid metabolism in bovine mammary-gland epithelial MAC-T cells after PKM2 knockdown.

Results

MAC-T cells stably expressing low levels of PKM2 were established with lentivirus-mediated small hairpin RNA. Although the knockdown of PKM2 had no effect on MAC-T cell growth, the reduced expression of PKM2 attenuated the mRNA and protein expression of key enzymes involved in sterol synthesis through the SREBP pathway.

Conclusions

The downregulation of PKM2 significantly influenced lipid synthesis in bovine mammary-gland epithelial MAC-T cells. These findings extend our understanding of the crosstalk between glycolysis and lipid metabolism in bovine mammary-gland epithelial cells.

Keywords

Bovine mammary-gland epithelial cells Lipid synthesis PKM2 SREBP 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31402158) and the Department of Henan Science and Technology (142300413209).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Bing-Qian Su
    • 1
  • Ying-Qian Han
    • 1
  • Shuang-Shuang Fan
    • 1
  • Sheng-Li Ming
    • 1
  • Bo Wan
    • 1
  • Wei-Fei Lu
    • 1
  • Bei-Bei Chu
    • 1
  • Guo-Yu Yang
    • 1
  • Jiang Wang
    • 1
  1. 1.College of Animal Sciences and Veterinary MedicineHenan Agricultural UniversityZhengzhouPeople’s Republic of China

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