Biotechnology Letters

, Volume 40, Issue 4, pp 689–696 | Cite as

A two-stage system coupling hydrolytic acidification with algal microcosms for treatment of wastewater from the manufacture of acrylonitrile butadiene styrene (ABS) resin

  • Shuhao Huo
  • Feifei Zhu
  • Bin Zou
  • Ling Xu
  • Fengjie Cui
  • Wenhua You
Original Research Paper



To demonstrate the effectiveness of a novel two-stage system coupling hydrolytic acidification with algal microcosms for the treatment of acrylonitrile butadiene styrene (ABS) resin-manufacturing wastewater.


After hydrolytic acidification, the BOD5/COD ratio increased from 0.22 to 0.56, showing improved biodegradability of the wastewater. Coupled with hydrolytic acidification, the algal microcosms showed excellent capability of in-depth removal of COD, NH3–N and phosphorus with removal rates 83, 100, and 89%, respectively, and aromatic pollutants, including benzene, were almost completely removed. The biomass concentration of Chlorella sp. increased from 5 × 106 to 2.1 × 107 cells/ml after wastewater treatment.


This two-stage coupling system achieved deep cleaning of the benzene-containing petrochemical wastewater while producing greater algae biomass resources at low cost.


Acrylonitrile butadiene styrene resin wastewater Algal microcosms Biodegradability Chlorella Hydrolytic acidification Microbial community 



This research was funded by the National Natural Science Foundation of China (21506084, 21406093, 31770394), the China Postdoctoral Science Foundation (2015T80502), the Senior Talent Scientific Research Initial Funding Project of Jiangsu University (14JDG024), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Training Project of the Young Core Instructor of Jiangsu University. We thank Edanz Editing China (, for editing the English text of a draft of this manuscript.

Supporting information

Supplementary Table 1—The quality of original ABS resin manufacturing wastewater.

Supplementary material

10529_2018_2513_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)


  1. Chavan A, Mukherji S (2010) Effect of co-contaminant phenol on performance of a laboratory-scale RBC with algal-bacterial biofilm treating petroleum hydrocarbon-rich wastewater. J Chem Technol Biotechnol 85:851–859CrossRefGoogle Scholar
  2. DOE (U.S. Department of Energy) (2010) National algal biofuels technology roadmap. U.S. Department of Energy, Energy Efficiency and Renewable Energy, Washington, DCGoogle Scholar
  3. Dou L (2011) Study on bio-electrochemical wastewater of ABS treatment technology and its mechanism. Hebei University of Engineering, HandanGoogle Scholar
  4. Hach (2008) Procedure manua. Hach, LovelandGoogle Scholar
  5. Hodges A, Fica Z, Wanlass J, VanDarlin J, Sims R (2017) Nutrient and suspended solids removal from petrochemical wastewater via microalgal biofilm cultivation. Chemosphere 174:46–48CrossRefPubMedGoogle Scholar
  6. Jauffraisa T, Agoguéc H, Gemina MP, Beaugeard L, Martin-Jézéquel V (2017) Effect of bacteria on growth and biochemical composition of two benthic diatoms Halamphora coffeaeformis and Entomoneis paludosa. J Exp Mar Biol Ecol 495:65–74CrossRefGoogle Scholar
  7. Kim W, Shin SG, Cho K, Lee C, Hwang S (2012) Performance of methanogenic reactors in temperature phased two-stage anaerobic digestion of swine wastewater. J Biosci Bioeng 114:635–639CrossRefPubMedGoogle Scholar
  8. Lai B, Zhou Y, Yang P, Wan K (2012) Comprehensive analysis of the toxic and refractory pollutants in acrylonitrile–butadiene–styrene resin manufacturing wastewater by gas chromatography spectrometry with a mass or flame ionization detector. J Chromatogr A 1244:161–167CrossRefPubMedGoogle Scholar
  9. Lu M, Zhang Z, Yu W, Zhu W (2009) Biological treatment of oilfield-produced water: a field pilot study. Int Biodeterior Biodegrad 63:316–321CrossRefGoogle Scholar
  10. Madadi R, Pourbabaee AA, Tabatabaei M, Zahed MA, Naghavi MR (2016) Treatment of petrochemical wastewater by the green algae Chlorella vulgaris. Int J Environ Res 10:555–560Google Scholar
  11. Ren N, Ding J, Chen Z (2012) High concentration industrial organic wastewater treatment technology. Chemical Industry Press, BeijingGoogle Scholar
  12. Schmidt PA, Balint M, Greshake B, Bandow C, Rombke J, Schmitt I (2013) Illumina metabarcoding of a soil fungal community. Soil Biol Biochem 65:128–132CrossRefGoogle Scholar
  13. Wang X, Zeng G, Zhu J (2008) Treatment of jean-wash wastewater by combined coagulation, hydrolysis/acidification and Fenton oxidation. J Hazard Mater 153(1–2):810–816CrossRefPubMedGoogle Scholar
  14. Wang K, Li W, Gong X, Li X, Liu W, He C, Wang Z, Minh QN, Chen C-L, Wang J-Y (2014) Biological pretreatment of tannery wastewater using a full-scale hydrolysis acidification system. Int Biodeterior Biodegrad 95:41–45CrossRefGoogle Scholar
  15. Zheng L (2017) Treatment of petrochemical wastewater through pulse hydrolysis acidification A/O process. Northeast Electric Power University, JilinGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Shuhao Huo
    • 1
  • Feifei Zhu
    • 2
  • Bin Zou
    • 1
  • Ling Xu
    • 1
  • Fengjie Cui
    • 1
  • Wenhua You
    • 3
  1. 1.School of Food and Biological EngineeringJiangsu UniversityZhenjiangChina
  2. 2.Institute of Life SciencesJiangsu UniversityZhenjiangChina
  3. 3.School of Environmental and Safety EngineeringJiangsu UniversityZhenjiangChina

Personalised recommendations