Biotechnology Letters

, Volume 40, Issue 4, pp 623–631 | Cite as

HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis

Original Research Paper
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Abstract

Objectives

To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer.

Results

HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung cancer cells. Importantly, HuR and CDK3 expressions were increased in lung cancer tissues compared with normal adjacent tissues, and positively correlated. Mechanistically, HuR directly bound to CDK3, and increased CDK3 mRNA stability and expression. Additionally, miR-873 or miR-125a-3p attenuated the promotion of HuR on CDK3 expression and lung cancer stemness. Furthermore, HuR facilitated lung cancer stemness dependent on CDK3 expression. miR-873 or miR-125a-3p level was negatively correlated with HuR and CDK3 expression levels in lung cancer tissues.

Conclusions

HuR facilitates lung cancer stemness via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis.

Keywords

CDK3 HuR Lung cancer miR-873 miR-125a-3p Stemness 

Notes

Supporting information

Supplementary Table 1—PCR primer sequences.

Compliance with ethical standards

Conflicts of interest

The authors declare no conflict of interest.

Supplementary material

10529_2018_2512_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Respiratory and Critical MedicineThe First Affiliated Hospital of Soochow UniversitySuzhouChina
  2. 2.Department of Respiratory MedicineWuxi No.9 People’s Hospital affiliated to Soochow UniversityWuxiChina

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