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Modeling and simulation of enzymatic gluconic acid production using immobilized enzyme and CSTR–PFTR circulation reaction system

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Abstract

Objectives

Production of gluconic acid by using immobilized enzyme and continuous stirred tank reactor-plug flow tubular reactor (CSTR–PFTR) circulation reaction system.

Results

A production system is constructed for gluconic acid production, which consists of a continuous stirred tank reactor (CSTR) for pH control and liquid storage and a plug flow tubular reactor (PFTR) filled with immobilized glucose oxidase (GOD) for gluconic acid production. Mathematical model is developed for this production system and simulation is made for the enzymatic reaction process. The pH inhibition effect on GOD is modeled by using a bell-type curve.

Conclusions

Gluconic acid can be efficiently produced by using the reaction system and the mathematical model developed for this system can simulate and predict the process well.

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References

  • Ateş S, İçli N (2013) Properties of immobilized glucose oxidase and enhancement of enzyme activity. Artif Cells Nanomed Biotechnol 41:264–268

    Article  PubMed  Google Scholar 

  • Christwardana M, Chung Y, Kwon Y (2017) Co-immobilization of glucose oxidase and catalase for enhancing the performance of a membraneless glucose biofuel cell operated under physiological conditions. Nanoscale 9:1993–2002

    Article  CAS  PubMed  Google Scholar 

  • Fiedurek J (2001) Production of gluconic acid by immobilized in pumice stones mycelium of Aspergillus niger using unconventional oxygenation of culture. Biotech Lett 23:1789–1792

    Article  CAS  Google Scholar 

  • Gao L, Ren Y, Ma Y, Lin J, Lin J (2011) Modeling and simulation of production of metallothionein and red fluorescent fusion protein by recombinant Escherichia Coli using graphical programming. In: Riccardo DA (ed) Modeling, programming and simulations using LabVIEW Software. Intech Press, Rijeka, pp 91–106

    Google Scholar 

  • Godjevargova T, Dayal R, Turmanova S (2004) Gluconic acid production in bioreactor with immobilized glucose oxidase plus catalase on polymer membrane adjacent to anion-exchange membrane. Macromol Biosci 4:950–956

    Article  CAS  PubMed  Google Scholar 

  • Guo Q, Liu G, Dong N, Lin J, Lin J (2013) Model predictive control of glucose feeding for fed-batch Candida utilis biomass production. Res J Biotechnol 8:3–7

    CAS  Google Scholar 

  • Hestekin JA, Lin YP, Frank JR (2002) Electrochemical enhancement of glucose oxidase kinetics: gluconic acid production with anion exchange membrane reactor. J Appl Electrochem 32:1049–1052

    Article  CAS  Google Scholar 

  • Idris S, Bakar AAA, Ratnam CT, Kamaruddin NH, Shaari S (2017) Influence of gamma irradiation on polymerization of pyrrole and glucose oxidase immobilization onto poly (pyrrole)/poly (vinyl alcohol) matrix. Appl Surf Sci 400:118–128

    Article  CAS  Google Scholar 

  • Kowalewska B, Jakubow K (2017) The impact of immobilization process on the electrochemical performance, bioactivity and conformation of glucose oxidase enzyme. Sens Actuator B-Chem 238:852–861

    Article  CAS  Google Scholar 

  • Lin J, Lee SM, Lee HJ, Koo YM (2000) Modeling of typical microbial cell growth in batch culture. Biotechnol Bioprocess Eng 5:382–385

    Article  CAS  Google Scholar 

  • Lin J, Lee SM, Koo YM (2004) Model development for lactic acid fermentation and parameter optimization using genetic algorithm. J Microbiol Biotechnol 14:26–27

    Google Scholar 

  • Lin Y, Liu G, Lin H, Gao L, Lin J (2013) Analysis of batch and repeated fedbatch productions of Candida utilis cell mass using mathematical modeling method. Electron J Biotechnol 16:6–8

    Google Scholar 

  • Lin J, Gao L, Lin H, Ren Y, Lin Y, Lin J (2017) Computer simulation of bioprocess. In: Cvetković D (ed) Computer simulation. Intech Press, Rijeka (in press)

    Google Scholar 

  • Milsom PE, Meers JL (1985) Gluconic acid, itaconic acid. In: Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology, 3rd edn. Pergamon, Oxford, pp 681–700

    Google Scholar 

  • Moxley MA, Beard DA, Bazil JN (2016) Global kinetic analysis of mammalian E3 Reveals pH-dependent NAD+/NADH regulation, physiological kinetic reversibility, and catalytic optimum. J Biol Chem 291:2712–2730

    Article  CAS  PubMed  Google Scholar 

  • Ozyilmaz G, Tukel SS, Alptekin O (2005) Activity and storage stability of immobilized glucose oxidase onto magnesium silicate. J Mol Catal B-Enzym 35:154–160

    Article  CAS  Google Scholar 

  • Shuler M, Kargu F (1992) Enzymes, in bioprocess engineering basic concepts. Prentice Hall inc, New Jersey

    Google Scholar 

  • Silva ARD, Tomotani EJ, Vitolo M (2011) Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors. Braz J Pharm Sci 47:399–407

    Article  Google Scholar 

  • Vanag VK, Míguez DG, Epstein IR (2006) Designing an enzymatic oscillator: bistability and feedback controlled oscillations with glucose oxidase in a continuous flow stirred tank reactor. J Chem Phys 125:194515

    Article  PubMed  Google Scholar 

  • Wang D, Wang C, Dong W, Shi J, Kim CH, Jiang B, Han Z, Jian H (2016) Gluconic acid production by gad mutant of Klebsiella pneumoniae. World J Microb Biotechnol 32:1–11

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by Shandong Province Science and Technology Development Project (2015GSF121016), the National Natural Science Foundation (61672329, 61602283), Shandong Natural Science Foundation (ZR2016FB10), and the State Key Laboratory of Microbial Technology Foundation of People’s Republic of China.

Supporting information

Supplementary 1.zip is the program of the simulation software.

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Authors and Affiliations

Authors

Contributions

CL, JL, and JL designed this study; CL and JL conducted the experiments; CL, JL, LG, HL, and JL analyzed the data; CL and JL wrote the paper. All the authors have read the manuscript critically.

Corresponding author

Correspondence to Jianqiang Lin.

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Conflict of interest

All the authors declare that there is no conflict of interest.

Ethical statement

The authors declare that there are no studies conducted with human participants or animals.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (ZIP 28 kb)

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Li, C., Lin, J., Gao, L. et al. Modeling and simulation of enzymatic gluconic acid production using immobilized enzyme and CSTR–PFTR circulation reaction system. Biotechnol Lett 40, 649–657 (2018). https://doi.org/10.1007/s10529-018-2509-4

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  • DOI: https://doi.org/10.1007/s10529-018-2509-4

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