Biotechnology Letters

, Volume 40, Issue 4, pp 649–657 | Cite as

Modeling and simulation of enzymatic gluconic acid production using immobilized enzyme and CSTR–PFTR circulation reaction system

  • Can Li
  • Jianqun Lin
  • Ling Gao
  • Huibin Lin
  • Jianqiang Lin
Original Research Paper



Production of gluconic acid by using immobilized enzyme and continuous stirred tank reactor-plug flow tubular reactor (CSTR–PFTR) circulation reaction system.


A production system is constructed for gluconic acid production, which consists of a continuous stirred tank reactor (CSTR) for pH control and liquid storage and a plug flow tubular reactor (PFTR) filled with immobilized glucose oxidase (GOD) for gluconic acid production. Mathematical model is developed for this production system and simulation is made for the enzymatic reaction process. The pH inhibition effect on GOD is modeled by using a bell-type curve.


Gluconic acid can be efficiently produced by using the reaction system and the mathematical model developed for this system can simulate and predict the process well.


Gluconic acid Immobilized glucose oxidase Modeling and simulation pH CSTR PFTR 



This work was supported by Shandong Province Science and Technology Development Project (2015GSF121016), the National Natural Science Foundation (61672329, 61602283), Shandong Natural Science Foundation (ZR2016FB10), and the State Key Laboratory of Microbial Technology Foundation of People’s Republic of China.

Supporting information

Supplementary is the program of the simulation software.

Author contributions

CL, JL, and JL designed this study; CL and JL conducted the experiments; CL, JL, LG, HL, and JL analyzed the data; CL and JL wrote the paper. All the authors have read the manuscript critically.

Compliance with ethical standards

Conflict of interest

All the authors declare that there is no conflict of interest.

Ethical statement

The authors declare that there are no studies conducted with human participants or animals.

Supplementary material (28 kb)
Supplementary material 1 (ZIP 28 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Technology, Microbiology Technology InstituteShandong UniversityJinanChina
  2. 2.Institute of Information Science and Engineering, School of Information Science and EngineeringShandong Normal UniversityJinanChina
  3. 3.Shandong Academy of Chinese MedicineJinanChina

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