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Biotechnology Letters

, Volume 40, Issue 1, pp 215–224 | Cite as

Tenocyte proliferation and migration promoted by rat bone marrow mesenchymal stem cell-derived conditioned medium

  • Qiufang Chen
  • Qingfei Liang
  • Weixia Zhuang
  • Jun Zhou
  • Bingyu Zhang
  • Pu Xu
  • Yang Ju
  • Yasuyuki Morita
  • Qing LuoEmail author
  • Guanbin SongEmail author
Original Research Paper

Abstract

Objectives

To investigate the impact of secreted factors of rat bone marrow mesenchymal stem cells (MSCs) on the proliferation and migration of tenocytes and provide evidence for the development of MSC-based therapeutic methods of tendon injury.

Results

Rat bone marrow mesenchymal stem cell-derived conditioned medium (MSC-CM) promoted the proliferation of tenocytes within 24 h and decreased the percentage of tenocytes in G1 phase. MSC-CM activated the extracellular signal-regulated kinase1/2 (ERK1/2) signal molecules, while the ERK1/2 inhibitor PD98059 abrogated the MSC-CM-induced proliferation of tenocytes, decreased the fraction of tenocytes in the G1 phase and elevated p-ERK1/2 expression. Furthermore, MSC-CM promoted the migration of tenocytes within 6 h, enhanced the formation of filamentous actin (F-actin) and increased the cellular and nuclear stiffness of tenocytes.

Conclusions

MSC-CM promotes tenocyte proliferation by changing cell cycle distribution via the ERK1/2 signaling pathway. MSC-CM-induced tenocyte migration was accompanied by cytoskeletal polymerization and increases in cellular and nuclear stiffness.

Keywords

Cytoskeleton Migration Mesenchymal stem cells Proliferation Stiffness Tenocytes 

Notes

Acknowledgements

This work was financially supported through grants from the Natural National Science Foundation of China (31700810, 11772073, 11272365 and 11532004), the exchange program of the National Natural Science Foundation of China and the Japan Society for the Promotion of Science (11511140092), the Chongqing Research Program of Basic Research and Frontier Technology (cstc2016jcyjA0222), and the Fundamental Research Funds for the Central Universities (106112017CDJXY230005).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Qiufang Chen
    • 1
  • Qingfei Liang
    • 1
  • Weixia Zhuang
    • 1
  • Jun Zhou
    • 1
  • Bingyu Zhang
    • 1
    • 2
  • Pu Xu
    • 1
  • Yang Ju
    • 3
  • Yasuyuki Morita
    • 3
  • Qing Luo
    • 1
    Email author
  • Guanbin Song
    • 1
    Email author
  1. 1.Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Ministry of EducationChongqing UniversityChongqingChina
  2. 2.Post-doctoral Mobile Stations of BiologyChongqing UniversityChongqingChina
  3. 3.Department of Mechanical Science and EngineeringNagoya UniversityNagoyaJapan

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