Tenocyte proliferation and migration promoted by rat bone marrow mesenchymal stem cell-derived conditioned medium
- 286 Downloads
To investigate the impact of secreted factors of rat bone marrow mesenchymal stem cells (MSCs) on the proliferation and migration of tenocytes and provide evidence for the development of MSC-based therapeutic methods of tendon injury.
Rat bone marrow mesenchymal stem cell-derived conditioned medium (MSC-CM) promoted the proliferation of tenocytes within 24 h and decreased the percentage of tenocytes in G1 phase. MSC-CM activated the extracellular signal-regulated kinase1/2 (ERK1/2) signal molecules, while the ERK1/2 inhibitor PD98059 abrogated the MSC-CM-induced proliferation of tenocytes, decreased the fraction of tenocytes in the G1 phase and elevated p-ERK1/2 expression. Furthermore, MSC-CM promoted the migration of tenocytes within 6 h, enhanced the formation of filamentous actin (F-actin) and increased the cellular and nuclear stiffness of tenocytes.
MSC-CM promotes tenocyte proliferation by changing cell cycle distribution via the ERK1/2 signaling pathway. MSC-CM-induced tenocyte migration was accompanied by cytoskeletal polymerization and increases in cellular and nuclear stiffness.
KeywordsCytoskeleton Migration Mesenchymal stem cells Proliferation Stiffness Tenocytes
This work was financially supported through grants from the Natural National Science Foundation of China (31700810, 11772073, 11272365 and 11532004), the exchange program of the National Natural Science Foundation of China and the Japan Society for the Promotion of Science (11511140092), the Chongqing Research Program of Basic Research and Frontier Technology (cstc2016jcyjA0222), and the Fundamental Research Funds for the Central Universities (106112017CDJXY230005).
- Hung SC, Pochampally RR, Chen SC, Hsu SC, Prockop DJ (2007) Angiogenic effects of human multipotent stromal cell conditioned medium activate the PI3 K-Akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis. Stem Cell 25:2363–2370CrossRefGoogle Scholar
- Ryan JA, Eisner EA, DuRaine G, You Z, Reddi AH (2009) Mechanical compression of articular cartilage induces chondrocyte proliferation and inhibits proteoglycan synthesis by activation of the ERK pathway: implications for tissue engineering and regenerative medicine. J Tissue Eng Regen Med 3:107–116CrossRefPubMedPubMedCentralGoogle Scholar