Biotechnology Letters

, Volume 39, Issue 12, pp 1827–1834 | Cite as

Non-small cell lung cancer: miR-30d suppresses tumor invasion and migration by directly targeting NFIB

  • Yubing Wu
  • Jingnan Zhang
  • Shizhen Hou
  • Ziming Cheng
  • Maoxi Yuan
Original Research Paper
  • 134 Downloads

Abstract

Objective

To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC).

Results

qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLCC patients. Over-expression of miR-30d notably inhibited cell migration and invasion in NSCLC cell lines. miR-30d could negatively regulate Nuclear factor I B (NFIB) by directly targeting its 3′-UTR, which was confirmed by luciferase assay. NFIB also reversed miR-30d-mediated suppression on the migration and invasion in NSCLC cell lines.

Conclusion

miR-30d suppressed cell migration and invasion by directly targeting NFIB in NSCLC, and NFIB could partially abrogated the inhibition of biological functions by miR-30d.

Keywords

Invasion miR-30d Migration Non-small cell lung cancer Nuclear factor I B 

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Yubing Wu
    • 1
  • Jingnan Zhang
    • 2
  • Shizhen Hou
    • 1
  • Ziming Cheng
    • 1
  • Maoxi Yuan
    • 1
  1. 1.Department of Thoracic SurgeryLinyi Central Hospital of ShandongLinyiPeople’s Republic of China
  2. 2.Department of PharmacyLinyi Central Hospital of ShandongLinyiPeople’s Republic of China

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