Fiber-type distribution and expression of myosin heavy chain isoforms in newborn heterozygous myostatin-knockout pigs
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To explore the effects of heterozygous myostatin-knockout (MSNT+/−) on muscle characteristics, specifically fiber-type distribution and expression of myosin heavy chain isoforms in pigs.
The fiber cross-sectional area of the semitendinosus and semimembranosus muscles were much larger in MSTN+/− pigs at birth than in wild-type (WT) pigs. MSTN+/− pigs had a higher proportion of fast-type fibers and lower succinate dehydrogenase activity in muscles than WT pigs. The myosin heavy chain IIB mRNA level in both two muscles was ~ threefold higher in MSTN+/− pigs compared with WT pigs.
MSTN+/− pigs exhibit a disproportionate increase in muscle mass and can have a higher body weight due to fiber hypertrophy, a change in the fiber-type distribution, and alteration of myosin heavy chain isoforms levels, leading to more fast glycolytic fibers.
KeywordsFast glycolytic fibers Heterozygous myostatin knockout Myosin heavy chain isoforms Myostatin Somatic cell nuclear transfer Transcription activator-like effector nuclease
This work was supported by the Science and Technology Development Projects of Jilin Province of China (Grant Number: 20160520057JH) and ToolGen, Inc. We thank the Hanji and Longxing pig farms for providing surrogate pig recipients and their enthusiasm and support for this research.
Supplementary Table 1—List of primers used for quantitative RT-PCR.
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