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Biotechnology Letters

, Volume 39, Issue 12, pp 1801–1810 | Cite as

LncRNA ZEB1-AS1 contributes to STAT3 activation by associating with IL-11 in B-lymphoblastic leukemia

  • Qing Wang
  • Xiaoli Du
  • Min Yang
  • Shishan Xiao
  • Jun Cao
  • Jun Song
  • Linhui Wang
Original Research Paper

Abstract

Objective

To investigate the role of lncRNA ZEB1-AS1 in B-lineage acute lymphoblastic leukemia (B-ALL).

Results

ZEB1-AS1 levels were aberrantly up-regulated in B-ALL. All correlated with STAT3 activation and IL-11 production. Moreover, a high level of ZEB1-AS1 predicted poor prognosis of B-ALL patients. Mechanistically, ZEB1-AS1 could bind to IL-11 and promote IL-11 stability. Down-regulation of ZEB1-AS1 decreased IL-11 production of human bone marrow stromal cells (BMSCs), which led to suppressed proliferation and inhibited IL-11/STAT3 pathway in BALL-1 cells.

Conclusions

ZEB1-AS1 promotes the activation of IL-11/STAT3 signaling pathway by associating with IL-11 in B-ALL.

Keywords

Acute lympho-blastic leukemia, IL-11 Long non-coding RNA, STAT3 ZEB1-AS1 

Notes

Acknowledgements

This work was supported by Departmental sources.

Supporting information

Supplementary Fig. 1—Expressions of ZEB1 and ZEB1-AS1.

Supplementary Fig. 2—Effects of ZEB1-AS1 knockdown in BALL-1 cells.

Supplementary Fig. 3—Effects of ZEB1-AS1 knockdown in HS-5 cells.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki Declaration and its later amendments.

Supplementary material

10529_2017_2421_MOESM1_ESM.doc (4.7 mb)
Supplementary material 1 (DOC 4824 kb)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Qing Wang
    • 1
  • Xiaoli Du
    • 1
  • Min Yang
    • 1
  • Shishan Xiao
    • 1
  • Jun Cao
    • 1
  • Jun Song
    • 1
  • Linhui Wang
    • 1
  1. 1.Department of HematologyGuizhou Provincial People’s HospitalGuiyangChina

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