Production of tartaric acid using immobilized recominant cis-epoxysuccinate hydrolase
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To investigate the expression and immobilization of recombinant cis-epoxysuccinate hydrolase (ESH), and its application in the biological production of l-(+)-tartaric acid.
E. coli BL21 (DE3)/pET11a-ESH (His) was engineered to express recombinant ESH. The enzyme had an activity of 262 U mg−1. The recombinant ESH was immobilized on agarose Ni-IDA matrix with metal ion affinity interaction to improve its thermostability and pH stability. The immobilization efficiency and activity yield were 94 and 95%, respectively. The specific catalytic efficiency of immobilized ESH was 104 mg U−1 h−1 during the continuous enzymatic production process.
ESH with a histidine tag was immobilized and used for the continuous production of l-(+)-tartaric acid.
KeywordsAffinity immobilization Enzymatic hydrolysis cis-Epoxysuccinate hydrolase l-(+)-Tartaric acid
This work was supported by the Major State Basic Research Development Program of China, Grant No. 2013CB733604.