Biotechnology Letters

, Volume 39, Issue 12, pp 1859–1863 | Cite as

Production of tartaric acid using immobilized recominant cis-epoxysuccinate hydrolase

  • Ziqiang Wang
  • Munan Su
  • Yanliang Li
  • Yunshan Wang
  • Zhiguo Su
Original Research Paper



To investigate the expression and immobilization of recombinant cis-epoxysuccinate hydrolase (ESH), and its application in the biological production of l-(+)-tartaric acid.


E. coli BL21 (DE3)/pET11a-ESH (His) was engineered to express recombinant ESH. The enzyme had an activity of 262 U mg−1. The recombinant ESH was immobilized on agarose Ni-IDA matrix with metal ion affinity interaction to improve its thermostability and pH stability. The immobilization efficiency and activity yield were 94 and 95%, respectively. The specific catalytic efficiency of immobilized ESH was 104 mg U−1 h−1 during the continuous enzymatic production process.


ESH with a histidine tag was immobilized and used for the continuous production of l-(+)-tartaric acid.


Affinity immobilization Enzymatic hydrolysis cis-Epoxysuccinate hydrolase l-(+)-Tartaric acid 



This work was supported by the Major State Basic Research Development Program of China, Grant No. 2013CB733604.


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Ziqiang Wang
    • 1
  • Munan Su
    • 1
  • Yanliang Li
    • 1
  • Yunshan Wang
    • 1
  • Zhiguo Su
    • 1
  1. 1.State Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingChina

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