Abstract
Objective
To study the effects of recombinant neuritin expressed by Pichia pastoris GS115 on the senescence, apoptosis, proliferation, and migration associated with rat bone marrow-derived mesenchymal stem cells (BMSCs).
Results
Recombinant neuritin was purified by Ni-affinity chromatography and identified by western blot and MALDI-TOF spectrometry. The effects of recombinant neuritin on senescence, apoptosis, proliferation, and migration of rat BMSCs WERE investigated. β-Galactosidase staining indicated that recombinant neuritin administration significantly inhibited BMSCs senescence at 1 μg neuritin/ml. Additionally, recombinant neuritin reduced the number of apoptotic cells at the early stage according to Annexin V/propidium iodide staining and inhibited cell proliferation according to MTT assay results. Moreover wound healing assay results showed that recombinant neuritin promoted BMSCs migration in the neuritin-treatment group.
Conclusion
Recombinant neuritin affects the senescence, apoptosis, proliferation, migration of rat BMSCs. Our findings offer insight into neuritin function outside of the nervous system.
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Acknowledgements
This study was supported by the Key Project of Xinjiang Province (Grant Nos. 2014AB048 and 2007JC 04) and Shihezi University High-Talents Funding (Grant No. RCZX200676). We would like to thank International Science Editing for English-language editing.
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Yuan, W., Cui, L., Li, G. et al. Recombinant neuritin affects the senescence, apoptosis, proliferation, and migration of rat bone marrow-derived mesenchymal stem cells. Biotechnol Lett 39, 1649–1655 (2017). https://doi.org/10.1007/s10529-017-2411-5
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DOI: https://doi.org/10.1007/s10529-017-2411-5