Recombinant neuritin affects the senescence, apoptosis, proliferation, and migration of rat bone marrow-derived mesenchymal stem cells
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To study the effects of recombinant neuritin expressed by Pichia pastoris GS115 on the senescence, apoptosis, proliferation, and migration associated with rat bone marrow-derived mesenchymal stem cells (BMSCs).
Recombinant neuritin was purified by Ni-affinity chromatography and identified by western blot and MALDI-TOF spectrometry. The effects of recombinant neuritin on senescence, apoptosis, proliferation, and migration of rat BMSCs WERE investigated. β-Galactosidase staining indicated that recombinant neuritin administration significantly inhibited BMSCs senescence at 1 μg neuritin/ml. Additionally, recombinant neuritin reduced the number of apoptotic cells at the early stage according to Annexin V/propidium iodide staining and inhibited cell proliferation according to MTT assay results. Moreover wound healing assay results showed that recombinant neuritin promoted BMSCs migration in the neuritin-treatment group.
Recombinant neuritin affects the senescence, apoptosis, proliferation, migration of rat BMSCs. Our findings offer insight into neuritin function outside of the nervous system.
KeywordsApoptosis BMSCs Cell viability Migration Neuritin Senescence
This study was supported by the Key Project of Xinjiang Province (Grant Nos. 2014AB048 and 2007JC 04) and Shihezi University High-Talents Funding (Grant No. RCZX200676). We would like to thank International Science Editing for English-language editing.