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Biotechnology Letters

, Volume 39, Issue 11, pp 1621–1629 | Cite as

miR-93 enhances hepatocellular carcinoma invasion and metastasis by EMT via targeting PDCD4

  • Chunmei Ji
  • Hang Liu
  • Qiang Yin
  • Hui Li
  • Han GaoEmail author
Original Research Paper

Abstract

Objectives

To clarify the potential biological function of miR-93 and its related molecular mechanism underlying metastasis in human hepatocellular carcinoma (HCC).

Results

miR-93 was significantly up-regulated in HCC tissues and was associated with poor 5-year overall survival in HCC patients. Transwell assays showed that over-expression of miR-93 increased HCC cell migration and invasion in vitro. Programmed cell death 4 (PDCD4) was a target gene of miR-93 and miR-93 could down-regulate the expression of PDCD4 by directly targeting its 3′-UTR. The re-expression of PDCD4 could attenuate the HCC cell invasion and migration induced by miR-93, while the knockdown of PDCD4 would promote HCC cell migration and invasion via the epithelial-mesenchymal transition (EMT) pathway.

Conclusions

miR-93 provides new insight into the molecular mechanisms of pathogenesis and progression in HCC and offer a potential therapeutic target for the treatment of HCC patients.

Keywords

Epithelial-mesenchymal transition (EMT) pathway Hepatocellular carcinoma Liver cancer metastasis miR-93 PDCD4 

Notes

Acknowledgements

None.

Compliance with ethical standards

Conflicts of interest

None.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Chunmei Ji
    • 1
  • Hang Liu
    • 2
  • Qiang Yin
    • 3
  • Hui Li
    • 4
  • Han Gao
    • 5
    Email author
  1. 1.Department of Clinical LaboratoryPeople’s Hospital of WeifangWeifangPeople’s Republic of China
  2. 2.Department of Interventional TherapyYidu Central Hospital of WeifangWeifangPeople’s Republic of China
  3. 3.Department of OncologyPeople‘s Hospital of RizhaoRizhaoPeople’s Republic of China
  4. 4.Department of PIVASPeople’s Hospital of ZhangqiuJinanPeople’s Republic of China
  5. 5.Department of PathologyQingdao Municipal HospitalQingdaoPeople’s Republic of China

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