Skip to main content

Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice

Biotechnology Letters volume 39, pages 1521–1527 (2017)Cite this article

Abstract

Objectives

To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions.

Results

The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%. Quantitative RT-PCR assays were performed to investigate the gene expression profiles of S. cerevisiae KKU-VN8. Differential expression of genes encoding heat-shock proteins (HSP82, HSP104, SSA4), genes involved in trehalose metabolism (TPS1, TPS2, NTH1) and genes involved the glycolytic pathway (ADH1, ADH2, CDC19) at various time points during fermentation was observed. The expression levels of HSP82, HSP104, SSA4, ADH1 and CDC19 were significantly higher than those of the controls (10.2-, 4-, 8-, 8.9- and 5.9-fold higher, respectively). In contrast, the expression levels of TPS1, TPS2, NTH1 and ADH2 were approx. 2-fold less than those of the controls.

Conclusions

The highly expressed genes encoding heat-shock proteins, HSP82 and SSA4, potentially play an important role in helping S. cerevisiae KKU-VN8 cope with various stresses that occur during high-temperature fermentation, leading to higher ethanol production efficiency.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  • Auesukaree C, Koedrith P, Saenpayavai P, Asvarak T, Benjaphokee S, Sugiyama M, Kaneko Y, Harashima S, Boonchird C (2012) Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. J Biosci Bioeng 114:144–149

    Article  CAS  PubMed  Google Scholar 

  • Charoensopharat K, Thanonkeo P, Thanonkeo S, Yamada M (2015) Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing. Antonie Van Leeuwenhoek 108:173–190

    Article  CAS  PubMed  Google Scholar 

  • Hauf J, Zimmermann FK, Müller S (2000) Simultaneous genomic overexpression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Enz Microb Technol 26:688–698

    Article  CAS  Google Scholar 

  • Hirasawa T, Yoshikawa K, Nakakura Y, Nagahisa K, Furusawa C, Katakura Y, Shimizu H, Shioya S (2007) Identification of target genes conferring ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis. J Biotechnol 131:34–44

    Article  CAS  PubMed  Google Scholar 

  • Hohmann S, Mager WH (2003) Yeast stress responses. Springer Science & Business Media, Berlin

    Book  Google Scholar 

  • Hou J, Jiao C, Peng B, Shen Y, Bao X (2016) Mutation of a regulator Ask10p improves xylose isomerase activity through up-regulation of molecular chaperones in Saccharomyces cerevisiae. Metab Eng 38:241–250

    Article  CAS  PubMed  Google Scholar 

  • Kim IS, Kim YS, Kim H, Jin I, Yoon HS (2013) Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation. Mol Cells 35:210–218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kitichantaropas Y, Boonchird C, Sugiyama M, Kaneko Y, Harashima S, Auesukaree C (2016) Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation. AMB Express 6:107

    Article  PubMed  PubMed Central  Google Scholar 

  • Limtong S, Sringiew C, Yongmanitchai W (2007) Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresour Technol 98:3367–3374

    Article  CAS  PubMed  Google Scholar 

  • Mahmud SA, Hirasawa T, Shimizu H (2010) Differential importance of trehalose accumulation in Saccharomyces cerevisiae in response to various environmental stresses. J Biosci Bioeng 109:262–266

    Article  CAS  PubMed  Google Scholar 

  • Nuanpeng S, Thanonkeo S, Yamada M, Thanonkeo P (2016) Ethanol production from sweet sorghum juice at high temperatures using a newly isolated thermotolerant yeast Saccharomyces cerevisiae DBKKU Y-53. Energies 9:253

    Article  Google Scholar 

  • Ogawa Y, Nitta A, Uchiyama H, Imamura T, Shimoi H, Ito K (2000) Tolerance mechanism of the ethanol-tolerant mutant of sake yeast. J Biosci Bioeng 90:313–320

    Article  CAS  PubMed  Google Scholar 

  • Patrascu E, Rapeanu G, Hopulele T (2009) Current approaches to efficient biotechnological production of ethanol. Innov Rom Food Biotechnol 4:1–11

    CAS  Google Scholar 

  • Techaparin A, Thanonkeo P, Klanrit P (2017) High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion. Braz J Microbiol 48:461–475

    Article  PubMed  PubMed Central  Google Scholar 

  • Xu X, Song H, Zhou Z, Shi N, Ying Q, Wang H (2010) Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress. Biotechnol Lett 32:979–987

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was financially supported by the National Research University Project of Thailand through the Biofuel Research Cluster of Khon Kaen University, Thailand (NRU 543042 and NRU-KKU-Ph.D 54310) and the Energy Conservation Fund of Energy Policy and Planning office (EPPO), Ministry of Energy, Thailand. We are grateful to the Fermentation Research Center for Value Added Agricultural Products (FerVAAP), the Department of Biotechnology, Faculty of Technology, Khon Kaen University and Molecular biology research laboratory, Faculty of Dentistry, Khon Kaen University for supporting the equipment and facilities used in this research.

Supporting information

Supplementary Table 1—Gene-specific primers.

Author information

Authors and Affiliations

  1. Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand

    Atiya Techaparin

  2. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand

    Atiya Techaparin, Pornthap Thanonkeo & Preekamol Klanrit

  3. Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen, 40002, Thailand

    Pornthap Thanonkeo & Preekamol Klanrit

Authors
  1. Atiya Techaparin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pornthap Thanonkeo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Preekamol Klanrit
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Preekamol Klanrit.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 23 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Techaparin, A., Thanonkeo, P. & Klanrit, P. Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. Biotechnol Lett 39, 1521–1527 (2017). https://doi.org/10.1007/s10529-017-2398-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-017-2398-y

Keywords

  • Gene expression
  • Glycolytic pathway
  • Heat-shock proteins
  • Saccharomyces cerevisiae
  • Sweet sorghum juice
  • Trehalose metabolism