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Bilirubin enzyme biosensor: potentiality and recent advances towards clinical bioanalysis

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Abstract

Bilirubin detection plays a major role in healthcare. Its high concentration in human serum is lethal and must be determined accurately. Clinically, it is vital for assessing patients with deleterious health conditions such as jaundice or icterus, hepatitis, mental disorders, cerebral palsy and brain damage especially in the case of neonates. In evaluating the drawbacks regarding the conventional methodology of bilirubin detection, there is need for a superior analytical tool. Bilirubin oxidase (BOx)-based sensors have been designed for the ultrasensitive analysis of bilirubin and quality deliverance of treatment and this review highlights the different mechanisms of bilirubin detection using different modified electrodes. Further, it also addresses the exploitation of highly attractive electrocatalytic properties of elite nanoparticles such as gold and zirconia- coated silica nanoparticles in enhancing the reproducibility and specificity of bilirubin biosensors.

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References

  • Andreu Y, Ostra M, Ubide C, Galbán J et al (2002) Study of a fluorometric-enzymatic method for bilirubin based on chemically modified bilirubin-oxidase and multivariate calibration. Talanta 57:343–353

    Article  CAS  PubMed  Google Scholar 

  • Avramescu ME, Sager WFC, Borneman Z, Wessling M (2004) Adsorptive membranes for bilirubin removal. J Chromatogr B 803:215–223

    Article  CAS  Google Scholar 

  • Baranano DE, Rao M, Ferris CD, Snyder SH (2002) Biliverdin reductase: a major physiologic cytoprotectant. Proc Natl Acad Sci USA 99:16093–16098

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Batra B, Lata S, Sunny Rana JS, Pundir CS (2013) Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film. Biosens Bioelectron 44:64–69

    Article  CAS  PubMed  Google Scholar 

  • Batra B, Malik N, Pundir CS (2015) Covalent immobilization of Myrothecium verrucaria bilirubin oxidase onto polypyrrole nanoparticles and polyaniline composite film for amperometric detection of serum bilirubin. Int J Curr Microbiol Appl Sci 4(7):875–888

    Google Scholar 

  • Bulmer AC, Ried K, Blanchfield JT, Wagner KH (2008) The anti-mutagenic properties of bile pigments. Mutat Res 658:28–41

    Article  CAS  PubMed  Google Scholar 

  • Chauhan N, Rawal R, Hooda V, Jain U (2016) Electrochemical biosensor with graphene oxide nanoparticles and polypyrrole interface for the detection of bilirubin. RSC Adv 6:63624–63633

    Article  CAS  Google Scholar 

  • Collinson MM (2007) Electrochemistry: an important tool to study and create new sol-gel-derived materials. Acc Chem Res 40:777–783

    Article  CAS  PubMed  Google Scholar 

  • Daniel MC, Astruc D (2003) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346

    Article  Google Scholar 

  • Dehghani H, Khoramnejadian S, Mahboubi M, Sasani M, Ghobadzadeh S, Haghighi SM, Negahdary M (2016) Bilirubin biosensing by using of catalase and ZnS nanoparticles as modifier. Int J Electrochem Sci 11:2029–2045

    CAS  Google Scholar 

  • Durand F, Gounel S, Kjaergaard CH, Solomon EI, Mano N (2012) Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications. Appl Microbiol Biotechnol 96:1489–1498

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ellairaja S, Shenbagavalli K, Ponmariappan S, Vasantha VS (2017) A green and facile approach for synthesizing imine to develop optical biosensor for wide range detection of bilirubin in human biofluids. Biosens Bioelectron 91:82–88

    Article  CAS  PubMed  Google Scholar 

  • Fortuney A, Guilbault G, Suleiman A (1996) Determination of bilirubin by fiberoptic biosensor. Anal Lett 29:171–180

    Article  Google Scholar 

  • Guo L, Chen G, Kim DH (2010) Three-dimensionally assembled gold nanostructures for plasmonic biosensors. Anal Chem 82:5147–5153

    Article  CAS  PubMed  Google Scholar 

  • Guo L, Ferhan AR, Lee K, Kim DH (2011) Nanoarray-based biomolecular detection using individual Au nanoparticles with minimized localized surface plasmon resonance variations. Anal Chem 83:2605–2612

    Article  CAS  PubMed  Google Scholar 

  • Hoshi T, Sagae N, Daikuhara K, Anzai JI (2007) Multilayer membranes via layer-by-layer deposition of ascorbate oxidase and Au nanoparticles on the Pt electrode for reduction of oxidation current derived from ascorbate. Talanta 71:644–647

    Article  CAS  PubMed  Google Scholar 

  • Jena BK, Raj CR (2006) Electrochemical biosensor based on integrated assembly of dehydrogenase enzymes and gold nanoparticles. Anal Chem 78:6332–6339

    Article  CAS  PubMed  Google Scholar 

  • Kannan P, John SA (2010) Highly sensitive determination of hydroxylamine using fused gold nanoparticles immobilized on sol-gel film modified gold electrode. Anal Chim Acta 663:158–164

    Article  CAS  PubMed  Google Scholar 

  • Kannan P, John SA (2011) Fabrication of conducting polymer-gold nanoparticles film on electrodes using monolayer protected gold nanoparticles and its electrocatalytic application. Electrochim Acta 56:7029–7037

    Article  CAS  Google Scholar 

  • Kannan P, Chen H, Lee VW, Kim DH (2011) Highly sensitive amperometric detection of bilirubin using enzyme and gold nanoparticles on sol-gel film modified electrode. Talanta 86:400–407

    Article  CAS  PubMed  Google Scholar 

  • Kaplan M, Hammerman C (2005) Understanding severe hyperbilirubinemia and preventing kernicterus: adjuncts in the interpretation of neonatal serum bilirubin. Clin Chim Acta 356:9–21

    Article  CAS  PubMed  Google Scholar 

  • Khan MM, Tayyab S (2001) Understanding the role of internal lysine residues of serum albumins in conformational stability and bilirubin binding. Biochim Biophys Acta 1545:263–277

    Article  CAS  PubMed  Google Scholar 

  • Klemm J, Prodromidis MI, Karayannis MI (2000) An enzymic method for the determination of bilirubin using an oxygen electrode. Electroanalysis 12:292–295

    Article  CAS  Google Scholar 

  • Léqer C, Bertrand P (2008) Direct electrochemistry of redox enzymes as a tool for mechanistic studies. Chem Rev 108:2379–2438

    Article  Google Scholar 

  • Li XP, Rosenzweig Z (1997) A fiber optic sensor for rapid analysis of bilirubin in serum. Anal Chim Acta 353:263–273

    Article  CAS  Google Scholar 

  • Liu Y, Li P, Lu J, Xiong W, Oger J, Tetzlaff W, Cynader M (2008) Bilirubin possesses powerful immunomodulatory activity and suppresses experimental autoimmune encephalomyelitis. J Immunol 181:1887–1897

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Du Y, Li CM (2013) Direct electrochemistry based biosensors and 365 biofuel cells enabled with nanostructured materials. Electroanalysis 25:815–823

    Article  CAS  Google Scholar 

  • Mano N (2012) Features and applications of bilirubin oxidases. Appl Microbiol Biotechnol 96:301–307

    Article  CAS  PubMed  Google Scholar 

  • Murata K, Kajiya K, Nakamura N, Ohno H (2009) Direct electrochemistry of bilirubin oxidase on three-dimensional gold nanoparticle electrodes and its application in a biofuel cell. Energy Environ Sci 2:1280–1285

    Article  CAS  Google Scholar 

  • Narang J, Chauhan N, Mathur A, Chaturvedi V, Pundir CS (2015) A third generation bilirubin sensor development by using gold nanomaterial as an immobilization matrix for signal amplification. Adv Mater Lett 6:1012–1017

    Article  CAS  Google Scholar 

  • Pita M, Gutierrez-Sanchez C, Toscano MD, Shleev S, De Lacey AL (2013) Third generation Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode. Bioelectrochem 94:69–74

    Article  CAS  Google Scholar 

  • Rahman MA, Lee KS, Park DS, Won MS, Shim YB (2008) An amperometric bilirubin biosensor based on a conductive poly-terthiophene-Mn(II) complex. Biosens Bioelectron 23:857–864

    Article  CAS  PubMed  Google Scholar 

  • Rakhi RB, Sethupathi K, Ramaprabhu S (2009) A glucose biosensor based on deposition of glucose oxidase onto crystalline gold nanoparticle modified carbon nanotube electrode. J Phys Chem B 113:3190–3194

    Article  CAS  PubMed  Google Scholar 

  • Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105:1547–1562

    Article  CAS  PubMed  Google Scholar 

  • Salaj-Kosla U, Scanlon MD, Baumeister T, Zahma K, Ludwig R, Conghaile PÓ, MacAodha D, Leech D, Magner E (2013) Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes. Anal Bioanal Chem 405:3823–3830

    Article  CAS  PubMed  Google Scholar 

  • Samanta S, Tan M, Kissack C, Nayak S, Chittick R, Yoxall CW (2004) The value of Bilicheck as a screening tool for neonatal jaundice in term and near-term babies. Acta Paediatr 93:1486–1490

    Article  CAS  PubMed  Google Scholar 

  • Santhosh M, Chinnadayyala SR, Kakoti A, Goswami P (2014) Selective and sensitive detection of free bilirubin in blood serum using human serum albumin stabilized gold nanoclusters as fluorometric and colorimetric probe. Biosens Bioelectron 59:370–376

    Article  CAS  PubMed  Google Scholar 

  • Saxena U, Chakraborty M, Goswami P (2011) Covalent immobilization of cholesterol oxidase on self-assembled gold nanoparticles for highly sensitive amperometric detection of cholesterol in real samples. Biosens Bioelectron 26:3037–3043

    Article  CAS  PubMed  Google Scholar 

  • Shipway AN, Katz E, Willner I (2000) Nanoparticle arrays on surfaces for electronic, optical, and sensor applications. Chem Phys Chem 1:18–52

    Article  CAS  PubMed  Google Scholar 

  • Shleev S, Kasmi A, Ruzgas T, Gorton L (2004) Direct heterogeneous electron transfer reactions of bilirubin oxidase at a spectrographic graphite electrode. Electrochem Commun 6:934–939

    Article  CAS  Google Scholar 

  • Shoham B, Migron Y, Riklin A, Willner I, Tartakovsky B (1995) A bilirubin biosensor based on a multilayer network enzyme electrode. Biosens Bioelectron 10:341–352

    Article  CAS  Google Scholar 

  • Si P, Kannan P, Guo L, Son H, Kim DH (2011) Highly stable and sensitive glucose biosensor based on covalently assembled high density Au nanostructures. Biosens Bioelectron 26:3845–3851

    Article  CAS  PubMed  Google Scholar 

  • Tanaka N, Murao S (1982) Purification and some properties of bilirubin oxidase of Myrothecium verrucaria MT-1. Agr Biol Chem 46:2499–2503

    CAS  Google Scholar 

  • Wang J, Ozsoz M (1990) A polishable amperometric biosensor for bilirubin. Electroanalysis 2:647–650

    Article  CAS  Google Scholar 

  • Wang X, Chowdhury JR, Chowdhury NR (2006) Bilirubin metabolism: applied physiology. Curr Paediatr 16:70–74

    Article  Google Scholar 

  • Wang C, Wang G, Fang B (2009) Electrocatalytic oxidation of bilirubin at ferrocenecarboxamide modified MWCNT–gold nanocomposite electrodes. Microchim Acta 164:113–118

    Article  CAS  Google Scholar 

  • Yang W, Wang J, Zhao S, Sun Y, Sun C (2006) Multilayered construction of glucose oxidase and gold nanoparticles on Au electrodes based on layer-by-layer covalent attachment. Electrochem Commun 8:665–672

    Article  CAS  Google Scholar 

  • Zhang S, Wang N, Yu H, Niu Y, Sun C (2005) Covalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor. Bioelectrochemistry 67:15–22

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Vinita Hooda, Anjum Gahlaut, Ashish Gothwal & Vikas Hooda

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  1. Vinita Hooda
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  2. Anjum Gahlaut
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  3. Ashish Gothwal
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  4. Vikas Hooda
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Correspondence to Vikas Hooda.

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Hooda, V., Gahlaut, A., Gothwal, A. et al. Bilirubin enzyme biosensor: potentiality and recent advances towards clinical bioanalysis. Biotechnol Lett 39, 1453–1462 (2017). https://doi.org/10.1007/s10529-017-2396-0

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  • DOI: https://doi.org/10.1007/s10529-017-2396-0

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