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Phytaspase-loaded, Mn-doped ZnS quantum dots when embedded into chitosan nanoparticles leads to improved chemotherapy of HeLa cells using in cisplatin

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Abstract

Objectives

To investigate the potential of recombinant phytaspase loaded manganese (Mn) doped zinc sulphide (ZnS) quantum dots embedded chitosan nanoparticles for augmenting cisplatin induced chemotherapy of HeLa cells.

Results

The recombinant phytaspase was cloned into bacterial expression vector PGEX-4T-2. The expressed and purified recombinant plant phytaspase protein from Escherichia coli BL21 was immobilized onto the cationic nanocomposite. Confocal microscopy elucidated the delivery of these luminescent nanocomposites inside cervical cancer HeLa cells. A 50% reduction in the viability of HeLa cells was achieved only in the case of phytaspase–nanocomposites–cisplatin combination at a dose of phytaspase (42 nM), nanocomposites (56.3 μg/ml) and cisplatin (0.44 μg/ml).

Conclusion

Luminescent cationic nanocomposites were developed for intracellular delivery of recombinant phytaspase, which due to its caspase-like activity assisted in substantiating the chemotherapeutic activity of apoptosis inducing drug-cisplatin.

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Acknowledgements

Financial supports from the Department of Biotechnology Programme Support, (BT/PR13560/COE/34/44/2015), DBT-NER/Health/47/2015) and the Department of Electronics and Information Technology (No. 5(9)/2012-NANO (Vol. II)), Government of India are acknowledged. Authors thank Centre for Nanotechnology and Central Instruments Facility (CIF), IIT Guwahati for providing instrument facility and Dr. Pallab Sanpui for his critical suggestions during the work.

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Authors and Affiliations

  1. Department of Biosciences and Bioengineering, Institute of Technology Guwahati, Guwahati, Assam, 781039, India

    Sharmila Narayanan, Neha Arora, Lingaraj Sahoo & Siddhartha Sankar Ghosh

  2. Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India

    Deepanjalee Dutta & Siddhartha Sankar Ghosh

Authors
  1. Sharmila Narayanan
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  2. Deepanjalee Dutta
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  3. Neha Arora
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  4. Lingaraj Sahoo
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  5. Siddhartha Sankar Ghosh
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Corresponding author

Correspondence to Siddhartha Sankar Ghosh.

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The authors declare that they have no conflict of interest.

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Narayanan, S., Dutta, D., Arora, N. et al. Phytaspase-loaded, Mn-doped ZnS quantum dots when embedded into chitosan nanoparticles leads to improved chemotherapy of HeLa cells using in cisplatin. Biotechnol Lett 39, 1591–1598 (2017). https://doi.org/10.1007/s10529-017-2395-1

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  • DOI: https://doi.org/10.1007/s10529-017-2395-1

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