Abstract
Objective
To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals.
Results
Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24–62%) and β-glucosidase (22–76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3.5–4.5 g l−1.
Conclusion
Enzyme recovery at regular intervals during fed-batch cellulase cultivation could protect the enzyme in the culture broth and enhance the enzyme production when the fungal cell concentration is maintained in a reasonable range.
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References
Ahamed A, Vermette P (2008a) Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions. Biochem Eng 40:399–407
Ahamed A, Vermette P (2008b) Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor. Biochem Eng J 42:41–46
Bailey MJ, Tähtiharju J (2003) Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy. Appl Microbiol Biotechnol 62:156–162
Cascalheira JF, Queiroz JA (1999) Kinetic study of the cellobiase activity of Trichoderma reesei cellulase complex at high substrate concentrations. Biotechnol Lett 21:651–655
Domingues FC, Queiroz JA, Cabral JMS, Fonseca LP (2001) Production of cellulases in batch culture using a mutant strain of Trichoderma reesei growing on soluble carbon source. Biotechnol Lett 23:771–775
dos Reis L, Fontana RC, Delabona Pda S, da Silva Lima DJ, Camassola M, Pradella JG, Dillon AJ (2013) Increased production of cellulases and xylanases by Penicillium echinulatum S1M29 in batch and fed-batch culture. Biores Technol 146:597–603
Gunjikar TP, Sawant SB, Joshi JB (2001) Shear deactivation of cellulase, exoglucanase, endoglucanase and β-glucosidase in a mechanically agitated reactor. Biotechnol Progr 17:1166–1168
He J, Yu B, Zhang K, Ding X, Chen D (2009) Strain improvement of Trichoderma reesei RUT C30 for increased cellulase production. Indian J Microbiol 49:188–195
Ju LK, Afolabi OA (1999) Wastepaper hydrolysate as soluble inducing substrate for cellulase production in continuous culture of Trichoderma reesei. Biotechnol Progr 15:91–97
Juhász T, Egyházi A, Réczey K (2005) β-Glucosidase production by Trichoderma reesei. Appl Biochem Biotechnol 121:243–254
Yu X, Yun H, Koo Y (1998) Production of cellulase by Trichoderma reesei RUT C30 in a batch fermentor. Microbiol Biotechnol 8:575–580
Zhang Q, Lo CM, Ju LK (2007) Factors affecting foaming behavior in cellulase fermentation by Trichoderma reesei RUT C30. Biores Technol 98:753–760
Acknowledgements
This work was supported by the National Key Technology Research and Development Program of China (2015BAD15B09).
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Wang, B., Jiang, L., Bai, H. et al. Regular enzyme recovery enhances cellulase production by Trichoderma reesei in fed-batch culture. Biotechnol Lett 39, 1493–1498 (2017). https://doi.org/10.1007/s10529-017-2379-1
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DOI: https://doi.org/10.1007/s10529-017-2379-1