MiR-9 enhances the sensitivity of A549 cells to cisplatin by inhibiting autophagy
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To demonstrate that miR-9 inhibits autophagy by down-regulating Beclin1 and thus enhances the sensitivity of A549 cells to cisplatin.
MiR-9 inhibited Beclin1 expression by binding to its 3′UTR. The inhibition decreased the cisplatin-induced autophagy in A549 cells, evidenced by the decreased expression of LC3II and GFP-LC3 puncta and the increased expression of P62. Upregulation of miR-9 level enhanced the sensibility of A549 cells to cisplatin and increased the cisplatin-induced apoptosis. Overexpression of Beclin1 reversed above effects of miR-9 mimics, cisplatin-induced autophagy was increased and apoptosis was decreased.
MiR-9 inhibits autophagy via targeting Beclin1 3′UTR and thus enhances cisplatin sensitivity in A549 cells.
KeywordsA549 cells Apoptosis Autophagy Beclin1 Cisplatin MiR-9
This study was funded by the National Major Special Program of New Drug Research and Development (No. 2013ZX09301303-005), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions and Qing Lan Project, National Natural Science Foundation of China, No. 81372331, and the Fundamental Research Funds for the Central Universities, No. 2015ZD004.
Supplementary Table 1—Primers of Beclin1 and GAPDH.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Fang D, Yang H, Lin J, Teng Y, Jiang Y, Chen J, Li Y (2015) 17beta-estradiol regulates cell proliferation, colony formation, migration, invasion and promotes apoptosis by upregulating miR-9 and thus degrades MALAT-1 in osteosarcoma cell MG-63 in an estrogen receptor-independent manner. Biochem Biophys Res Commun 457:500–506CrossRefPubMedGoogle Scholar