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Biotechnology Letters

, Volume 39, Issue 7, pp 959–966 | Cite as

MiR-9 enhances the sensitivity of A549 cells to cisplatin by inhibiting autophagy

  • Yan Zhang
  • Xia Meng
  • Cheng Li
  • Zhoulin Tan
  • Xinwei Guo
  • Zhiting Zhang
  • Tao Xi
Original Research Paper

Abstract

Objectives

To demonstrate that miR-9 inhibits autophagy by down-regulating Beclin1 and thus enhances the sensitivity of A549 cells to cisplatin.

Results

MiR-9 inhibited Beclin1 expression by binding to its 3′UTR. The inhibition decreased the cisplatin-induced autophagy in A549 cells, evidenced by the decreased expression of LC3II and GFP-LC3 puncta and the increased expression of P62. Upregulation of miR-9 level enhanced the sensibility of A549 cells to cisplatin and increased the cisplatin-induced apoptosis. Overexpression of Beclin1 reversed above effects of miR-9 mimics, cisplatin-induced autophagy was increased and apoptosis was decreased.

Conclusions

MiR-9 inhibits autophagy via targeting Beclin1 3′UTR and thus enhances cisplatin sensitivity in A549 cells.

Keywords

A549 cells Apoptosis Autophagy Beclin1 Cisplatin MiR-9 

Notes

Acknowledgements

This study was funded by the National Major Special Program of New Drug Research and Development (No. 2013ZX09301303-005), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions and Qing Lan Project, National Natural Science Foundation of China, No. 81372331, and the Fundamental Research Funds for the Central Universities, No. 2015ZD004.

Supporting information

Supplementary Table 1—Primers of Beclin1 and GAPDH.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10529_2017_2325_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Yan Zhang
    • 1
    • 2
  • Xia Meng
    • 1
    • 2
  • Cheng Li
    • 1
    • 2
  • Zhoulin Tan
    • 1
    • 2
  • Xinwei Guo
    • 1
    • 2
  • Zhiting Zhang
    • 1
    • 2
  • Tao Xi
    • 1
    • 2
  1. 1.School of Life Science and TechnologyChina Pharmaceutical UniversityNanjingPeople’s Republic of China
  2. 2.Jiangsu Key Laboratory of Carcinogenesis and InterventionChina Pharmaceutical UniversityNanjingPeople’s Republic of China

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