CDK inhibitor SU9516 induces tetraploid blastocyst formation from parthenogenetically activated porcine embryos
To examine the effect of SU9516, a cyclin-dependent kinase inhibitor, on the induction of tetraploid blastocyst formation in porcine embryos by parthenogenetic activation.
Karyotype analysis of blastocysts showed that in the SU9516-treatment group 56% were tetraploid, whereas in the cytochalasin B (CB) group 67% were diploid. The level of maturation-promoting factor (MPF) in stimulated embryos treated with 10 µM SU9516 for 4 h was lower than in embryos treated with CB group (103 vs. 131 pg/ml). The mRNA expression levels of Nanog significantly increased in SU9516-treated embryos than CB group.
SU9516 can induce tetraploid blastocyst formation at high efficiency. SU9516 can significantly influence the in vitro developmental competence of porcine parthenogenetically activated embryos by influencing the level of MPF and the gene related apoptosis and pluripotency.
KeywordsEmbryos Maturation-promoting factor Pigs Polar body Parthenogenetic activation SU9516 Tetraploid embryos
This work was supported by the State Key Development Program for Basic Research of China (Grant No. 20150622005JC) and the institute for Basic Science (Grant No. IBS-R021-D1-2015-a02). Guo Qing is greatly indebted to his thesis supervisor, Dr. Yin Xi-Jun and Dr.Kang Jin-Dan.
Supplementary Table 1—Primer sequences used for gene expression analysis.
- Ulloa Ulloa CM, Yoshizawa M, Yamashita A, Hama S, Mitsui A, Hashi C, Abe H, Hoshi H, Fukui E, Matsumoto H (2008) Blastocyst production from in vitro-produced day-2 bovine embryos classified by cleavage stage, and cytogenetical evaluation of the resultant day-8 blastocysts. J Reprod Dev 54:465–472CrossRefPubMedGoogle Scholar