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Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease through the regulation of 15-LOX-1 in macrophages

Abstract

Objective

To investigate the role of human umbilical cord mesenchymal stem cells (hucMSCs) in the treatment of dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD).

Results

ICG-hucMSCs homed to colon tissues of IBD mice 12 h after injection. The injection of hucMSCs significantly relieved the IBD symptoms and inflammatory cell infiltration. The expression of IL-10 gene increased while those of 15-LOX-1, TNF-α, IL-6, IL-1β, and IP-10 genes decreased in colon tissues and spleens of hucMSCs-treated mice. The activation of STAT3 was inhibited in colon tissues and spleens of IBD mice that were treated with hucMSCs. In addition, the percentage of macrophages decreased in colon tissues and spleens of hucMSCs-treated IBD mice. Moreover, we provided evidence that in vitro co-culture with hucMSCs inhibited the expression of 15-LOX-1, IL-6 and p-STAT3 in mouse enterocoelia macrophages.

Conclusions

HucMSCs alleviate DSS-induced IBD through the modulation of 15-LOX-1 in macrophages.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (Grant No. 81670502, 81672416, 81272481), Jiangsu Key Research and Development Project (Grant No. BE2016717), Jiangsu Province for Outstanding Sci-tech Innovation Team in Colleges and Universities (SJK2013-10), the China postdoctoral science foundation (Grant No. 2015M571705), the Natural Science Foundation of Jiangsu Province for high schools (Grant No. 10KJB310002), Jiangsu Province’s scientific and technological Supporting Program (Grant No. BE2010703), the Scientific Research Foundation of Jiangsu University (Grant No. 10JDG094, FCJJ2015023), the opening project of the Key Laboratory of Embryo Molecular Biology, Ministry of Health of China, and Shanghai Key Laboratory of Embryo and Reproduction Engineering (Grant No. KF201601), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Supporting information

Supplementary Table 1—Primer sequences for qRT-PCR.

Author information

Correspondence to Wenrong Xu.

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The authors declare that they have no conflict of interest.

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Cite this article

Mao, F., Wu, Y., Tang, X. et al. Human umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease through the regulation of 15-LOX-1 in macrophages. Biotechnol Lett 39, 929–938 (2017). https://doi.org/10.1007/s10529-017-2315-4

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Keywords

  • hucMSCs
  • Inflammatory bowel disease
  • Macrophages
  • Mesenchymal stem cells 15-LOX-1