Biotechnology Letters

, Volume 39, Issue 4, pp 577–587

A novel, versatile family IV carboxylesterase exhibits high stability and activity in a broad pH spectrum

  • Amélie Dukunde
  • Dominik Schneider
  • Mingji Lu
  • Silja Brady
  • Rolf Daniel
Original Research Paper

DOI: 10.1007/s10529-016-2282-1

Cite this article as:
Dukunde, A., Schneider, D., Lu, M. et al. Biotechnol Lett (2017) 39: 577. doi:10.1007/s10529-016-2282-1
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Abstract

Objectives

To investigate the properties of a novel metagenome-derived member of the hormone-sensitive lipase family of lipolytic enzymes.

Results

A forest soil metagenome-derived gene encoding an esterase (Est06) belonging to the hormone-sensitive lipase family of lipolytic enzymes was subcloned, heterologously expressed and characterized. Est06 is a polypeptide of 295 amino acids with a molecular mass of 31 kDa. The deduced protein sequence shares 61% similarity with a hypothetical protein from the marine symbiont Candidatus Entotheonella sp. TSY1. Purified Est06 exhibited high affinity for acyl esters with short-chain fatty acids, and showed optimum activity with p-nitrophenyl valerate (C5). Maximum enzymatic activity was at 50 °C and pH 7. Est06 exhibited high stability at moderate temperatures by retaining all of its catalytic activity below 30 °C over 13 days. Additionally, Est06 displayed high stability between pH 5 and 9. Esterase activity was not inhibited by metal ions or detergents, although organic solvents decreased activity.

Conclusions

The combination of Est06 properties place it among novel biocatalysts that have potential for industrial use including low temperature applications.

Keywords

Carboxylesterases Esterase Family IV esterase Hormone-sensitive lipase family Soil metagenome 

Supplementary material

10529_2016_2282_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1333 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and GeneticsGeorg-August University GöttingenGöttingenGermany
  2. 2.Bayer AGET-TD-UP Biochemistry & BiocatalysisLeverkusenGermany

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