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Promigratory and proangiogenic effects of AdipoRon on bone marrow-derived mesenchymal stem cells: an in vitro study

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Abstract

Objectives

To investigate the effect of AdipoRon on major factors involved in survival, migration and neovascularization of rat bone marrow-derived mesenchymal stem cells.

Results

AdipoRon promoted the MSCs viability. Real-time PCR indicated that the expression of cyclooxygenase-2 (COX-2), hypoxia-inducible factor-1 (HIF-1) C-X-C chemokine receptor type 4 (CXCR4), C–C chemokine receptor type 2 (CCR2), vascular endothelial growth factor matrix metalloproteinase-2 (MMP-2) and MMP-9 were upregulated in AdipoRon-treated MSCs compared to control groups. Prostaglandin E2 (PGE2) level, as well as migration ability of MSCs (scratch assay) was enhanced by AdipoRon preconditioning.

Conclusion

Preconditioning of MSCs with AdipoRon prior to transplantation could enhance cell survival, angiogenesis and migration via activating the COX-2/PGE2/HIF-1 pathway and other contributing factors.

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Acknowledgments

This study was supported by a grant from Hamadan University of Medical Sciences and Iranian Council of Stem Cell Technology.

Supporting information

Supplementary Table 1—Primer sequences used in the quantitative Real-time PCR.

Supplementary Fig. 1—Characterization of rat MSCs.

Author information

Authors and Affiliations

  1. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Sara Malih, Massoud Saidijam, Mona Pourjafar, Maryam Sadat Tafakh, Fahimeh Talebzadeh & Rezvan Najafi

  2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Kamran Mansouri

  3. Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Rezvan Najafi

Authors
  1. Sara Malih
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  2. Massoud Saidijam
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  3. Kamran Mansouri
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  4. Mona Pourjafar
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  5. Maryam Sadat Tafakh
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  6. Fahimeh Talebzadeh
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  7. Rezvan Najafi
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Corresponding author

Correspondence to Rezvan Najafi.

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Conflict of interest

The authors declare they have no conflict of interests.

Ethical approval

This study was approved by Ethics Committee of Hamadan University of Medical Sciences based on National Institutes of Health Principles of Laboratory Animal Care (NIH Publication No. 85–23, revised 1985).

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Supplementary material 1 (DOCX 70 kb)

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Malih, S., Saidijam, M., Mansouri, K. et al. Promigratory and proangiogenic effects of AdipoRon on bone marrow-derived mesenchymal stem cells: an in vitro study. Biotechnol Lett 39, 39–44 (2017). https://doi.org/10.1007/s10529-016-2214-0

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  • DOI: https://doi.org/10.1007/s10529-016-2214-0

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