Abstract
Objectives
To analyze the anti-insect mechanism of viral pesticide AcMNPV-BmK IT(P10/PH) in the host Spodoptera frugiperda 9 (Sf9) cells.
Results
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)- mediated expression of BmK IT, regulated by P10 protein promoter (P10) and polyhedrosis promoter (PH), promoted the replication of progeny virus in host Sf9 cells. AcMNPV-BmK IT(P10) could accelerate the budding process (or speed) of budded virus (BV) in Sf9 cells. The impact of AcMNPV-BmK IT(P10) on the nuclear polymerization of filamentous actin (F-actin) participated in regulating the accelerated budding process. Unexpectedly, both AcMNPV-BmK IT(P10) and AcMNPV-BmK IT(PH) delayed the nuclear polymerization of F-actin and promoted the clearance of F-actin in the nucleus. SfP53, an important apoptosis factor, was involved in the regulation of AcMNPV-BmK IT(P10/PH) in Sf9 cells. AcMNPV-BmK IT(P10/PH) could also delay and promote the nuclear recruitment of SfP53 after 27 h post infection (h p.i.).
Conclusion
SfP53 and F-actin are the targets of viral pesticide AcMNPV-BmK IT (P10/PH) in host Sf9 cells, which provides the experimental basis for the development of recombinant baculovirus biopesticides.
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Acknowledgments
This project was supported by Grants from ‘National Natural Science Foundation of China (No. 31272100 and 31372199)’, ‘Natural Science Foundation of Shanxi Province (No. 2014011038-1)’.
Supporting Information
Supplementary Table 1—Sequence of primers used to amplify vp39, polh and β-actin gene.
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Leixi Cao and Xing Li have contributed equally to this work.
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Cao, L., Li, X., Zheng, S. et al. SfP53 and filamentous actin (F-actin) are the targets of viral pesticide AcMNPV-BmK IT (P10/PH) in host Spodoptera frugiperda 9 cells. Biotechnol Lett 38, 2059–2069 (2016). https://doi.org/10.1007/s10529-016-2201-5
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DOI: https://doi.org/10.1007/s10529-016-2201-5