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MicroRNA-145-based differentiation of human mesenchymal stem cells to smooth muscle cells

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Abstract

Objectives

To investigate the role of microRNA-145, that regulates gene expression of genes related to differentiation, proliferation and the phenotype of smooth muscle cells (SMCs), in the differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) to SMCs.

Results

Real-time PCR analysis indicated significant upregulation of SMC markers, including SM-α-actin, calponin, caldesmon and SMMHC, in SMCs compared to hBM-MSCs. Conversely, Krüppel-like factor 4, the direct target of microRNA-145 and the suppressor of smooth muscle differentiation, was suppressed in hBM-MSC-derived SMCs. Western blot analysis and immunocytochemistry also confirmed that the introduction of microRNA-145 into hBM-MSCs induced mature contractile SMCs. The functionality of hBM-MSC-derived SMCs was assessed by proliferation assay using PDGF-BB and contractility assay using carbachol. The results showed that the produced SMCs contracted in response to carbachol stimulation.

Conclusion

Overexpression of microRNA-145 in undifferentiated hBM-MSCs results in functionally mature contractile SMCs that can be used in drug discovery and cell therapy in SMC disorders such as vascular disease.

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Acknowledgments

The authors acknowledge the support from the Research Council of Tarbiat Modares University and Stem Cell Technology Research Center (Tehran, Iran) for providing the necessary facilities and funds for this research work.

Supporting Information

Supplementary Table 1—Primer sequences used in the quantitative real-time PCR.

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

    Maryam Pajoohesh & Hossein Naderi-Manesh

  2. Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran

    Maryam Pajoohesh

  3. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

Authors
  1. Maryam Pajoohesh
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  2. Hossein Naderi-Manesh
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  3. Masoud Soleimani
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Corresponding author

Correspondence to Hossein Naderi-Manesh.

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The authors declare no financial or commercial conflict of interest.

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Pajoohesh, M., Naderi-Manesh, H. & Soleimani, M. MicroRNA-145-based differentiation of human mesenchymal stem cells to smooth muscle cells. Biotechnol Lett 38, 1975–1981 (2016). https://doi.org/10.1007/s10529-016-2177-1

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  • DOI: https://doi.org/10.1007/s10529-016-2177-1

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