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Organic two-photon nanoparticles modulate reactive oxygen species, intracellular calcium concentration, and mitochondrial membrane potential during apoptosis of human gastric carcinoma SGC-7901 cells

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Abstract

Objectives

To investigate the biocompatibility of human gastric carcinoma cells (SGC-7901) with organic two-photon nanoparticles (NPs).

Results

Different concentrations of NPs were incubated with SGC-7901 cells for different times. The levels of cell apoptosis, reactive oxygen species (ROS), intracellular calcium, and mitochondrial membrane potential (MMP) were measured by staining the SGC-7901 cells with Annexin V-FITC/PI, 2′,7′-dichlorofluorescin diacetate, Fluo-3 AM, and Rhodamine 123, followed by the flow cytometry assay. NPs at <4 µg/ml, did not have any significant effect on apoptosis, necrosis, generation of ROS, increase of intracellular Ca2+ concentration or decrease of MMP in SGC-7901 cells, but >4 µg/ml had a major effects on all the above mentioned parameters.

Conclusion

2,5,2′,5′-Tetra(4-N,N-diphenylamine styryl) biphenyl NPs can be used at an appropriate concentration as a safe drug carrier or imaging marker and may serve as an effective tool for developing a photodynamic cancer therapy.

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Acknowledgments

This study was financially supported by the National Science Foundation of China (No. 51173066).

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Authors and Affiliations

  1. Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China

    Geng Chen & Lian-Kun Sun

  2. Department of Gastroenterology, The First Hospital of Jilin University, No. 71 XinMin Street, Changchun, 130021, China

    Geng Chen, Shou-Ying Li, Hamid Tayyab Malik & Hong Xu

  3. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130021, China

    Yu-Guang Ma

Authors
  1. Geng Chen
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  2. Shou-Ying Li
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  3. Hamid Tayyab Malik
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  4. Yu-Guang Ma
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  5. Hong Xu
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  6. Lian-Kun Sun
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Corresponding authors

Correspondence to Hong Xu or Lian-Kun Sun.

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Chen, G., Li, SY., Malik, H.T. et al. Organic two-photon nanoparticles modulate reactive oxygen species, intracellular calcium concentration, and mitochondrial membrane potential during apoptosis of human gastric carcinoma SGC-7901 cells. Biotechnol Lett 38, 1269–1276 (2016). https://doi.org/10.1007/s10529-016-2105-4

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  • DOI: https://doi.org/10.1007/s10529-016-2105-4

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