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Impact of transduction towards the proliferation and migration as well as the transduction efficiency of human umbilical cord-derived late endothelial progenitor cells with nine recombinant adeno-associated virus serotypes

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Abstract

Objectives

To evaluate the transduction efficiency of human umbilical cord-derived, late endothelial progenitor cells late (HUCB-late EPCs) with nine recombinant adeno-associated virus (rAAV) serotypes and the ability of proliferation and migration of the cells after transduction.

Results

rAAV2 and rAAV6 showed a greater ability than other serotypes to transduce late EPCs (P < 0.05). After transduction, cell proliferation ability weakened (P < 0.05), but the ability of migration to stromal cell-derived factor (SDF-1) unchanged.

Conclusion

There is an advantage of choosing the optimal rAAV serotype as a gene vector to alter the biologic characteristics of late EPCs.

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Acknowledgments

The authors thank Deng Xiyun and Cao Jianguo for their insightful comments and careful review of the manuscript.

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Author notes

    Authors and Affiliations

    1. Department of Cardiology of Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410000, China

      Zhaofei Wang, Qiang Fu & Zhaofen Zheng

    2. The Medical College of Hunan Normal University, Changsha, 410000, China

      Jianguo Cao, Xiyun Deng & Yue Li

    3. The Medical College of Nanhua University, Hengyang, 421001, China

      Qiaofeng Wang

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    1. Zhaofei Wang
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    3. Jianguo Cao
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    Corresponding author

    Correspondence to Zhaofen Zheng.

    Additional information

    Zhaofei Wang and Qiang Fu contributed equally to this work.

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    Wang, Z., Fu, Q., Cao, J. et al. Impact of transduction towards the proliferation and migration as well as the transduction efficiency of human umbilical cord-derived late endothelial progenitor cells with nine recombinant adeno-associated virus serotypes. Biotechnol Lett 38, 1073–1079 (2016). https://doi.org/10.1007/s10529-016-2082-7

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    • DOI: https://doi.org/10.1007/s10529-016-2082-7

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