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Secreted expression of truncated capsid protein from porcine circovirus type 2 in Pichia pastoris

Biotechnology Letters volume 38, pages 959–967 (2016)Cite this article

Abstract

Objective

To achieve secreted expression of the truncated capsid protein from porcine circovirus type 2 (PCV2) in Pichia pastoris.

Results

A truncated cap gene (tcap) with a deleted N-terminal nuclear localization signal was optimized and synthesized. Effective secreted expression was achieved in P. pastoris GS115. The high-productive recombinant strain for tCap was grown in a 5 l bioreactor and the productivity of tCap in supernatant reached 250 μg/ml. Furthermore, serum antibody test demonstrated that adjuvant-assisting tCap induced a significant increase of specific PCV2-Cap antibody over time in mice and a similar antibody level in pigs compared with a commercial Cap-based subunit vaccine.

Conclusion

This work establishes a secreted expression strategy in P. pastoris for the production of PCV2 Cap with superior bioactivity, and this strategy might provide potential uses in developing Cap-based subunit vaccine in the future.

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Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, 200237, China

    Xiaohong Chen, Junjun Chen, Yuanxing Zhang & Qin Liu

  2. Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China

    Yuanxing Zhang & Qin Liu

  3. Beijing Wanmuyuan Agriculture S&T Co., Ltd, Beijing, 100081, China

    Ping Zhu

  4. China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Beijing, 100081, China

    Yong Deng

Authors
  1. Xiaohong Chen
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  2. Junjun Chen
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  3. Yuanxing Zhang
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  4. Ping Zhu
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  5. Yong Deng
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  6. Qin Liu
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Correspondence to Yong Deng or Qin Liu.

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Chen, X., Chen, J., Zhang, Y. et al. Secreted expression of truncated capsid protein from porcine circovirus type 2 in Pichia pastoris . Biotechnol Lett 38, 959–967 (2016). https://doi.org/10.1007/s10529-016-2076-5

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  • DOI: https://doi.org/10.1007/s10529-016-2076-5

Keywords

  • Immunogenicity
  • Pichia pastoris
  • Porcine circovirus diseases
  • Porcine circovirus type 2
  • Secretion expression
  • Truncated capsid protein