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Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production

Biotechnology Letters volume 38, pages 975–982 (2016)Cite this article

Abstract

Objectives

To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated.

Results

Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta-ackA strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation.

Conclusions

K. pneumoniae knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity.

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Acknowledgments

This work was supported by the Industrial Strategic Technology Development Program (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) and the Advanced Biomass R&D Center of Global Frontier Project (No. ABC-2010-0029728) funded by the Ministry of Science, ICT and Future Planning (MSIP, Korea).

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Affiliations

  1. Research and Development Center, GS Caltex Corporation, Expo-ro 359, Yusung-gu, Daejeon, 305-380, Republic of Korea

    Chelladurai Rathnasingh, Jong Myoung Park, Duk-ki Kim & Hyohak Song

  2. BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yusung-gu, Daejeon, 305-704, Republic of Korea

    Duk-ki Kim & Yong Keun Chang

Authors
  1. Chelladurai Rathnasingh
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  2. Jong Myoung Park
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  3. Duk-ki Kim
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  4. Hyohak Song
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  5. Yong Keun Chang
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Corresponding author

Correspondence to Hyohak Song.

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Rathnasingh, C., Park, J.M., Kim, Dk. et al. Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production. Biotechnol Lett 38, 975–982 (2016). https://doi.org/10.1007/s10529-016-2062-y

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  • DOI: https://doi.org/10.1007/s10529-016-2062-y

Keywords

  • Acetate kinase
  • Alcohol dehydrogenase
  • 2,3-Butanediol
  • In silico investigation
  • Klebsiella pneumoniae
  • Lactate dehydrogenase
  • Phosphotransacetylase