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Biotransformation of hexavalent chromium into extracellular chromium(III) oxide nanoparticles using Schwanniomyces occidentalis

Biotechnology Letters volume 38, pages 441–446 (2016)Cite this article

Abstract

Objectives

To demonstrate biotransformation of toxic Cr(VI) ions into Cr2O3 nanoparticles by the yeast Schwanniomyces occidentalis.

Results

Reaction mixtures containing S. occidentalis NCIM 3459 and Cr(VI) ions that were initially yellow turned green after 48 h incubation. The coloration was due to the synthesis of chromium (III) oxide nanoparticles (Cr2O3NPs). UV–Visible spectra of the reaction mixtures showed peaks at 445 and 600 nm indicating 4A2g → 4T1g and 4A2g → 4T2g transitions in Cr2O3, respectively. FTIR profiles suggested the involvement of carboxyl and amide groups in nanoparticle synthesis and stabilization. The Cr2O3NPs ranged between 10 and 60 nm. Their crystalline nature was evident from the selective area electron diffraction and X-ray diffraction patterns. Energy dispersive spectra confirmed the chemical composition of the nanoparticles. These biogenic nanoparticles could find applications in different fields.

Conclusions

S. occidentalis mediated biotransformation of toxic Cr(VI) ions into crystalline extracellular Cr2O3NPs under benign conditions.

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Acknowledgments

PM wishes to thank the Council of Scientific and Industrial Research, India for Senior Research Fellowship. All authors thank University Grants Commission, India for support under University with Potential for Excellence Phase II.

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Authors and Affiliations

  1. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, 411007, India

    Pallavi T. Mohite, Ameeta Ravi Kumar & Smita S. Zinjarde

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  1. Pallavi T. Mohite
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  2. Ameeta Ravi Kumar
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  3. Smita S. Zinjarde
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Correspondence to Smita S. Zinjarde.

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Mohite, P.T., Kumar, A.R. & Zinjarde, S.S. Biotransformation of hexavalent chromium into extracellular chromium(III) oxide nanoparticles using Schwanniomyces occidentalis . Biotechnol Lett 38, 441–446 (2016). https://doi.org/10.1007/s10529-015-2009-8

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  • DOI: https://doi.org/10.1007/s10529-015-2009-8

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