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Metabolic engineering of Saccharomyces cerevisiae for linalool production

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Abstract

Objectives

To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool.

Results

Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its native promoter with the repressible MET3 promoter in the presence of methionine resulted in accumulation of 78 µg linalool l−1 in the culture medium. This was more than twice that produced by the control strain. The highest linalool titer was obtained by combined repression of ERG9 and overexpression of tHMG1. The yeast strain harboring both modifications produced 95 μg linalool l−1.

Conclusions

Although overexpression of tHMG1 and downregulation of ERG9 enhanced linalool titers threefold in the engineered yeast strain, alleviating linalool toxicity is necessary for further improvement of linalool biosynthesis in yeast.

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Acknowledgments

This study was supported by a grant (No. 89/84468) from the Biotechnology Committee, University of Isfahan, Iran. The authors are grateful to Prof. Wilfried Schwab, Technical University of Munich, for the kind gift of plasmid bearing linalool synthase gene from Lavandula angustifolia. Dr. Hadi Parastar is also thanked for assistance with GC analysis.

Supporting Information

Supplementary Table 1—Primers used for the PCR amplifications.

Author information

Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Pegah Amiri & Azar Shahpiri

  2. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran

    Mohammad Ali Asadollahi

  3. Department of Analytical Chemistry, Faculty of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran

    Fariborz Momenbeik

  4. Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada

    Siavash Partow

Authors
  1. Pegah Amiri
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  2. Azar Shahpiri
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  3. Mohammad Ali Asadollahi
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  4. Fariborz Momenbeik
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  5. Siavash Partow
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Corresponding author

Correspondence to Mohammad Ali Asadollahi.

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Supplementary material 1 (DOCX 11 kb)

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Amiri, P., Shahpiri, A., Asadollahi, M.A. et al. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett 38, 503–508 (2016). https://doi.org/10.1007/s10529-015-2000-4

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  • DOI: https://doi.org/10.1007/s10529-015-2000-4

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