Abstract
Objective
To explore the metabolic process of Paenibacillus shenyangensis that is an efficient bioflocculant-producing bacterium. The biosynthesis mechanism of bioflocculation was used to enrich the genome of Paenibacillus shenyangensis and provide a basis for molecular genetics and functional genomics analyses.
Results
According to the analysis of de novo assembly, a total of 5,501,467 bp clean reads were generated, and were assembled into 92 contigs. 4800 unigenes were predicted of which 4393 were annotated showing a specific gene function in the NCBI-Nr database. 3423 genes were found in the database of cluster of orthologous groups. Among the 168 Kyoto Encyclopedia of Genes and Genomes database, cell growth and metabolism were the main biological processes, and a potential metabolic pathway was predicted from glucose to exopolysaccharide within the starch and sucrose metabolism pathway.
Conclusion
By using the high-throughput sequencing technology, we provide a genome analysis of Paenibacillus shenyangensis that predicts the main metabolic processes and a potential pathway of exopolysaccharide biosynthesis.
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Acknowledgments
This research was supported by the National Natural Science Foundation of China (No. 51278090), the Major Science and Technology Program for Water Pollution Control and Treatment (Nos. 2012ZX07202-004-06, 013ZX07202-010-05), and the Fundamental Research Funds for the Central Universities of China (No. N120601003). Thanks are also due to Pengcheng Yan, Wei Peng and Beijing Computing Center for technical support.
Supporting information
Supplementary Fig. 1: Metabolic pathways of Paenibacillus shenyangensis (a. Citrate cycle, b. Glycolysis, c. Pentose phosphate pathway, d. Amino acid pathways, e. Starch and sucrose metabolism pathway.
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Fu, L., Jiang, B., Liu, J. et al. Genome sequence analysis of a flocculant-producing bacterium, Paenibacillus shenyangensis . Biotechnol Lett 38, 447–453 (2016). https://doi.org/10.1007/s10529-015-1990-2
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DOI: https://doi.org/10.1007/s10529-015-1990-2