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A cold-adapted and glucose-stimulated type II α-glucosidase from a deep-sea bacterium Pseudoalteromonas sp. K8

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Abstract

Objectives

To express and characterize a putative α-glucosidase, Pagl, from Pseudoalteromonas sp. K8 obtained via genome mining approach.

Results

Pagl was expressed and purified to homogeneity, with a molecular mass of 60 kDa. It was optimally active at pH 8.5 and 30 °C, and showed cold-adapted activity. Pagl exhibited specific activity towards substrates with α-1,4-linkage, with the highest specific activity of 19.4 U/mg for maltose, followed by pNPαG and maltodextrins, suggesting that Pagl belongs to the type II α-glucosidase. Interestingly, the activity of Pagl is significantly enhanced (2.7 times) in the presence of 200 mM glucose.

Conclusion

The unique catalytic properties of Pagl make it an attractive candidate for several industrial applications.

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Acknowledgments

This work was supported by Grants from the National Natural Science Foundation of China (31570064); the Natural Science Foundation of the Department of Education of Anhui Province (KJ2015A038); the Key Scientific and Technological project of Anhui Province (1301032154); the Introduction Project of Academic and Technology Leaders in Anhui University (32030066) and the Innovative Research Team Program of 211 Project in Anhui University.

Supporting information

Supplementary Table 1—Primers used in cloning pagl from Pseudoalteromonas sp. K8.

Supplementary Table 2—Effects of cations on Pagl activitya.

Supplementary Fig. 1—Kinetic profile of Pagl under concentrations of glucose.

Author information

Authors and Affiliations

  1. School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China

    Wei Li, Yi Xue, Jingjing Li, Jing Yuan, Wei Fang, Zemin Fang & Yazhong Xiao

  2. Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China

    Wei Li, Yi Xue, Jingjing Li, Jing Yuan, Wei Fang, Zemin Fang & Yazhong Xiao

  3. Department of Chemistry & Biochemistry, Florida International University, Miami, FL, 33199, USA

    Xiaotang Wang

Authors
  1. Wei Li
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  2. Yi Xue
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  3. Jingjing Li
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  4. Jing Yuan
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  5. Xiaotang Wang
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  6. Wei Fang
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  7. Zemin Fang
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  8. Yazhong Xiao
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Corresponding author

Correspondence to Zemin Fang.

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Li, W., Xue, Y., Li, J. et al. A cold-adapted and glucose-stimulated type II α-glucosidase from a deep-sea bacterium Pseudoalteromonas sp. K8. Biotechnol Lett 38, 345–349 (2016). https://doi.org/10.1007/s10529-015-1987-x

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  • DOI: https://doi.org/10.1007/s10529-015-1987-x

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