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Fusion expression of cecropin B-like antibacterial peptide in Pichia GS115 and its antibacterial mechanism

Biotechnology Letters volume 38, pages 305–312 (2016)Cite this article

Abstract

Objectives

To establish an efficient expression system for a fusion protein of glutathione S-transferase and cecropin B (GST-CB) and to clarify the antibacterial mechanism of CB.

Results

The optimal incubation time and methanol concentration for induced expression of CB were 36 h and 1 % w/v, respectively. The yield of GST-CB was 2.2 g/l. The minimum inhibitory concentrations of GST-CB towards Staphylococcus aureus subsp. saprophyticus (ATCC 15305) and Escherichia coli strain CFT073 were 250 and 125 μg/ml, respectively. Notably, mutations of proline 24 (P24) in CB produced a polypeptide without antimicrobial activity.

Conclusion

The fusion protein GST-CB, which has a broad spectrum antimicrobial activity, can be abundantly expressed in Pichia pastoris GS115, and P24 may be an important amino acid for the antimicrobial activity of GST-CB.

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Acknowledgments

This work was supported by Innovation Team Project of the Ministry of Education of China (IRT0959-208).

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Authors and Affiliations

  1. Key Laboratory of Dairy Science Ministry of Education, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin, 150030, China

    Wei Yu, Xue Jun Gao, Ying Liu & Qi Wang

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  1. Wei Yu
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  2. Xue Jun Gao
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  3. Ying Liu
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  4. Qi Wang
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Correspondence to Xue Jun Gao.

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Yu, W., Gao, X.J., Liu, Y. et al. Fusion expression of cecropin B-like antibacterial peptide in Pichia GS115 and its antibacterial mechanism. Biotechnol Lett 38, 305–312 (2016). https://doi.org/10.1007/s10529-015-1978-y

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  • DOI: https://doi.org/10.1007/s10529-015-1978-y

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