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Deletion of arcA increased the production of acetyl-CoA-derived chemicals in recombinant Escherichia coli

An Erratum to this article was published on 01 March 2016

Abstract

Objective

Acetyl-CoA is used to produce many valuable metabolites in Escherichia coli. However, acetate overflow is a major shortcoming. Knockout of the global regulator gene, arcA, may solve this problem.

Results

The arcA gene of E. coli BL21(DE3) was knocked out, and the production of phloroglucinol (PG) and 3-hydroxypropionate (3HP), both derived from acetyl-CoA, were used to evaluate its effect. The arcA mutants had higher cell yields and higher glucose utilization efficiencies than the corresponding control strains, and the productions of PG and 3HP were 0.92 g/l and 0.27 g/l, respectively; more than twice that of the control strains. Furthermore, arcA knockout also showed significant repression on formation of acetate, the major byproduct in fermentation. Acetate concentrations were decreased 69.4 % and 87 % by arcA knockout during the production of PG and 3HP, respectively.

Conclusions

The arcA gene knockout is a solution to acetate overflow and may improve production of a wide range of acetyl-CoA-derived metabolites.

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Acknowledgments

This work was supported by100-Talent Project of CAS (to GZ), Natural Science Foundation of Shandong Province (ZR2013EMZ002), National Natural Science Foundation of China (21376255), Taishan Scholars Climbing Program of Shandong(No.tspd20150210)and Key Program of CAS (KGZD-EW-606-1-3).

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Correspondence to Huizhou Liu or Gao Zhao.

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Liu, M., Yao, L., Xian, M. et al. Deletion of arcA increased the production of acetyl-CoA-derived chemicals in recombinant Escherichia coli . Biotechnol Lett 38, 97–101 (2016). https://doi.org/10.1007/s10529-015-1953-7

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  • DOI: https://doi.org/10.1007/s10529-015-1953-7

Keywords

  • Acetate-derived metabolites
  • Acetyl-CoA
  • arcA
  • Escherichia coli
  • 3-Hydroxypropionate
  • Phloroglucinol