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Enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from Cellulosimicrobium cellulans

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Abstract

Objectives

To find extracellular biocatalysts that can specifically and efficiently remove the C-7 xylosyl group from 7-xylosyltaxanes.

Results

A Cellulosimicrobium cellulans strain F16 that can remove the C-7 xylosyl group from 7-xylosyltaxanes was isolated from the root soil of an old Taxus yunnanensis tree. Using corn cob as sole carbon source, the maximum 7-xylosyl-10-deacetylpaclitaxel β-xylosidase activity of 9.6 U l−1 was achieved. The β-xylosidase could be trapped by a ceramic tubular membrane (pore size 50 nm), and exhibited an apparent molecular weight much greater than 500 kDa. Under optimized conditions, 3.75 l cell-free culture medium transformed 2 grams 7-xylosyltaxane mixtures to their corresponding aglycones within 3 h, with a conversion >98 %.

Conclusion

This is the first report that C. cellulans can produce extracellular β-xylosidases capable of removing the C-7 xylosyl group from 7-xylosyltaxanes.

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References

  • Cheng HL, Zhao RY, Chen TJ, Yu WB, Wang F, Cheng KD, Zhu P (2013) Cloning and characterization of the glycoside hydrolases that remove xylosyl groups from 7-beta-xylosyl-10-deacetyltaxol and its analogues. Mol Cell Proteomics 12:2236–2248

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ge GB, Liang SC, Hu Y, Liu XB, Mao YX, Zhang YY, Luan HW, Qiu MH, Yang L (2010) Rapid qualitative and quantitative determination of seven valuable taxanes from various taxus species by UFLC-ESI-MS and UFLC-DAD. Planta Med 76:1773–1777

    Article  CAS  PubMed  Google Scholar 

  • Georgiev MI, Weber J (2014) Bioreactors for plant cells: hardware configuration and internal environment optimization as tools for wider commercialization. Biotechnol Lett 36:1359–1367

    Article  CAS  PubMed  Google Scholar 

  • Hanson RL, Howell JM, Brzozowski DB, Sullivan SA, Patel RN, Szarka LJ (1997) Enzymic hydrolysis of 7-xylosyltaxanes by xylosidase from Moraxella sp. Biotechnol Appl Biochem 26:153–158

    CAS  Google Scholar 

  • Jordan MA, Wilson L (2004) Microtubules as a target for anticancer drugs. Nat Rev Cancer 4:253–265

    Article  CAS  PubMed  Google Scholar 

  • Ludwig-Muller J (2015) Plants and endophytes: equal partners in secondary metabolite production? Biotechnol Lett. doi:10.1007/s10529-015-1814-4

    PubMed  Google Scholar 

  • McNeil MM, Brown JM, Carvalho ME, Hollis DG, Morey RE, Reller LB (2004) Molecular epidemiologic evaluation of endocarditis due to Oerskovia turbata and CDC group A-3 associated with contaminated homograft valves. J Clin Microbiol 42:2495–2500

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Patel RN (1998) Tour de paclitaxel: biocatalysis for semisynthesis. Annu Rev Microbiol 52:361–395

    Article  CAS  PubMed  Google Scholar 

  • Tian J, Stella VJ (2008) Degradation of paclitaxel and related compounds in aqueous solutions I: epimerization. J Pharm Sci 97:1224–1235

    Article  CAS  PubMed  Google Scholar 

  • Wang K, Wang T, Li J, Zou J, Chen Y, Dai J (2011a) Microbial hydrolysis of 7-xylosyl-10-deacetyltaxol to 10-deacetyltaxol. J Mol Catal B 68:250–255

    Article  CAS  Google Scholar 

  • Wang XH, Zhang C, Yang LL, Li S, Zhang Y, Gomes-Laranjo J (2011b) Screening and identification of microbial strains that secrete an extracellular C-7 xylosidase of taxanes. World J Micro Biol 27:627–635

    Article  Google Scholar 

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2009AA02Z205), the National Natural Science Foundation of China (No. 81102345) and the National Key Technology Research and Development Program of China (2009BADB9B02).

Conflict of interest

The authors declare that they have no conflict of interest.

Supporting information

Supplementary Figure 1 scanning electron microscopy results. a Grown on corn cob for 18 hours; b Grown on corn cob for 48 hours. Bar, 5 μm

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    Authors and Affiliations

    1. Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457, Zhongshan Road, Dalian, 116023, Liaoning, People’s Republic of China

      Tong-Yi Dou, Hong-Wei Luan, Xing-Bao Liu, Shi-Yang Li, Xun-Fu Du & Ling Yang

    2. University of the Chinese Academy of Sciences, Beijing, People’s Republic of China

      Tong-Yi Dou

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    1. Tong-Yi Dou
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    5. Xun-Fu Du
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    Corresponding author

    Correspondence to Ling Yang.

    Additional information

    Tong-Yi Dou and Hong-Wei Luan have contributed equally to this work.

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    Dou, TY., Luan, HW., Liu, XB. et al. Enzymatic hydrolysis of 7-xylosyltaxanes by an extracellular xylosidase from Cellulosimicrobium cellulans . Biotechnol Lett 37, 1905–1910 (2015). https://doi.org/10.1007/s10529-015-1867-4

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    • DOI: https://doi.org/10.1007/s10529-015-1867-4

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