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Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress

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Abstract

Objective

To investigate whether genes other than the operons nahAaAbAcAdBFCED and nahGTHINLOMKJ of Pseudomonas putida are involved in the tolerance of the bacterium to naphthalene.

Results

Cellular responses of P. putida ND6 grown with 2 and 4 g naphthalene/l were investigated using a quantitative proteomic-based approach. Comparative analysis of the proteome data identified that the expression levels of 22 proteins involved in heat shock and universal stress response, naphthalene degradation, cell envelope synthesis, and motility were up-regulated; while the expression levels of 26 proteins involved in protein and fatty acid synthesis, carbon compound, nucleotide, and amino acid metabolism, and small molecule transport were down-regulated.

Conclusion

Our findings offer insights into the cellular response of P. putida to high naphthalene concentrations at the protein level.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 21037002 and 21477163) and the Tianjin Research Program of Application Foundation and Advanced Technology (No. 14JCZDJC40400).

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Authors and Affiliations

  1. Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Logistics University of Chinese People’s Armed Police Forces, Hui-Zhi Ring Road 1, Tianjin, 300309, People’s Republic of China

    Shan-Shan Li, Xiao Hu, Hong Zhao, Ya-Xiao Li, Li Zhang, Li-Jun Gong, Jin Guo & Hua-Bing Zhao

  2. Department of Environmental Science and Engineering, Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China

    Shan-Shan Li

  3. Animals, Plants and Food Testing Center of Tianjin Exit-Entry Inspection & Quarantine Bureau, Tianjin, 300461, People’s Republic of China

    Hua-Bing Zhao

Authors
  1. Shan-Shan Li
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  2. Xiao Hu
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  3. Hong Zhao
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  4. Ya-Xiao Li
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  5. Li Zhang
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  6. Li-Jun Gong
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  7. Jin Guo
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  8. Hua-Bing Zhao
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Corresponding author

Correspondence to Hua-Bing Zhao.

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Li, SS., Hu, X., Zhao, H. et al. Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress. Biotechnol Lett 37, 1645–1654 (2015). https://doi.org/10.1007/s10529-015-1828-y

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  • DOI: https://doi.org/10.1007/s10529-015-1828-y

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