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Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery

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Abstract

Objectives

The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme.

Results

We hypothesized that use of such hyperthermophilic enzymes would allow for the biofuel cells to have a long battery life. Using pyrroloquinoline quinone-glucose dehydrogenase and the multicopper oxidase from Pyrobaculum aerophilum, we constructed an anode and cathode. The maximum output was 11 μW at 0.2 V, and the stability of the both electrode was maintained at 70 % after 14 days.

Conclusion

The biofuel cells that use hyperthermophilic enzymes may prolong their life.

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Acknowledgments

This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (23510127) Grant-in-Aid for Scientific Research (C).

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Authors and Affiliations

  1. Tenure-Track Program for Innovative Research, University of Fukui, Fukui, Japan

    Hiroaki Sakamoto

  2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan

    Toshiki Uchii, Kayo Yamaguchi, Takenori Satomura & Shin-ichiro Suye

  3. Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan

    Ayako Koto, Ei-ichiro Takamura & Shin-ichiro Suye

  4. Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kagawa, Japan

    Haruhiko Sakuraba

  5. Department of Biomedical Engineering, Faculty of Engineering, Osaka Institute of Technology, Osaka, Japan

    Toshihisa Ohshima

Authors
  1. Hiroaki Sakamoto
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  2. Toshiki Uchii
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  3. Kayo Yamaguchi
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  4. Ayako Koto
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  5. Ei-ichiro Takamura
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  6. Takenori Satomura
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  7. Haruhiko Sakuraba
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  8. Toshihisa Ohshima
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  9. Shin-ichiro Suye
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Corresponding author

Correspondence to Shin-ichiro Suye.

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Sakamoto, H., Uchii, T., Yamaguchi, K. et al. Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery. Biotechnol Lett 37, 1399–1404 (2015). https://doi.org/10.1007/s10529-015-1819-z

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  • DOI: https://doi.org/10.1007/s10529-015-1819-z

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