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Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst

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Abstract

Objective

To produce 10-ketostearic acid from oleic acid.

Results

Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l−1 and 2.5 g oleic acid l−1. Under these conditions, the cells produced 1.96 g 10-ketostearic acid l−1 from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l−1 h−1.

Conclusion

This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.

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Acknowledgments

This work was supported by the MOTIE/KEIT R&D Program (10044604, Bioproduction of long-chain dicarboxylic acids from fatty acids and lipids).

Supporting information

Supplementary Table 1—Bacterial strains, plasmids and oligonucleotides used in this study.

Author information

Author notes

    Authors and Affiliations

    1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea

      Byeonghun Lee, Saebom Lee, Hyeonsoo Kim & Jinwon Lee

    2. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon, 305-701, Republic of Korea

      Kijun Jeong

    3. Department of Food Science and Engineering, Ewha Womans University, Seoul, 120-750, Republic of Korea

      Jinbyung Park

    4. Department of Chemical Engineering, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea

      Eunyeol Lee

    Authors
    1. Byeonghun Lee
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    2. Saebom Lee
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    7. Jinwon Lee
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    Corresponding author

    Correspondence to Jinwon Lee.

    Additional information

    Byeonghun Lee and Saebom Lee have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOCX 17 kb)

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    Lee, B., Lee, S., Kim, H. et al. Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst. Biotechnol Lett 37, 1101–1106 (2015). https://doi.org/10.1007/s10529-015-1775-7

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    • DOI: https://doi.org/10.1007/s10529-015-1775-7

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