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Highly sensitive fluorescence biosensors for sparfloxacin detection at nanogram level based on electron transfer mechanism of cadmium telluride quantum dots

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Abstract

A sensitive fluorescence biosensor for determining sparfloxacin (SPF) based on the electron transfer mechanism and the fluorescence quenching effect of SPF to cadmium telluride quantum dots (CdTe QDs) was developed. The mechanism of the interaction between SPF and CdTe QDs was investigated by UV/Vis absorption and fluorescence spectroscopy. The biosensor could be used for the determination of SPF with a high sensitivity. Under optimum conditions, the linear range was from 0.28 to 40 μg SPF ml−1 with a correlation coefficient of 0.9983, and the detection limit (3δ/k) was 83.7 ng SPF ml−1. Furthermore, this method has been applied to the determination of SPF in the synthetic environmental water samples and the spiked human serum samples with good results.

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Acknowledgments

This work was supported by Chongqing Municipal Key Laboratory on Luminescence and Real-Time Analysis, Southwest University (CSTC, 2006CA8006) and the National Natural Science Foundation of China (No. 21175015).

Supporting information

Supplementary Fig. 1—Molecular structure of sparfloxacin.

Supplementary Fig. 2—a Effect of pH on the fluorescence intensity of the solution system in the presence (F) and the absence (F0) of sparfloxacin (SPF, c SPF: 5 μg SPF ml−1, cQDs: 5 × 10−5 M) and b effect of incubation time (c SPF: 5 μg SPF ml−1, cQDs: 5 × 10−5 M).

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Authors and Affiliations

  1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China

    Wanjun Liang, Shaopu Liu, Jing Song, Chenxia Hao, Linlin Wang, Dan Li & Youqiu He

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  1. Wanjun Liang
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  2. Shaopu Liu
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  3. Jing Song
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  4. Chenxia Hao
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  5. Linlin Wang
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  6. Dan Li
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  7. Youqiu He
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Correspondence to Youqiu He.

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Liang, W., Liu, S., Song, J. et al. Highly sensitive fluorescence biosensors for sparfloxacin detection at nanogram level based on electron transfer mechanism of cadmium telluride quantum dots. Biotechnol Lett 37, 1057–1061 (2015). https://doi.org/10.1007/s10529-015-1767-7

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  • DOI: https://doi.org/10.1007/s10529-015-1767-7

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