Abstract
We described a new method to label biological molecules using gold nanoparticles (GNPs) and double stranded DNA. Researchers can conveniently label their own samples with GNPs using this method. The label is based on dsDNA with a 93.5 % coverage of GNPs (dsDNA:GNP = 303:1). Antigens, streptavidin and biotin were labeled on GNPs and the success of the method was investigated with agarose gel electrophoresis, laser particle size analysis and ultraviolet spectrophotometry. These analyses confirmed that biological molecules were successfully bound to the GNPs. These molecules retained their biological activity and were able to detect targets on PVDF and NC membranes with excellent selectivity and low levels of background. Modified GNPs were also able to detect targets on nylon membranes, but with some degree of false positives. The maximum limit of detection was 25 ng proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bouzas V, Haller T, Hobi N, Felder E, Pastoriza-Santos I, Perez-Gil J (2014) Nontoxic impact of PEG-coated gold nanospheres on functional pulmonary surfactant-secreting alveolar type II cells. Nanotoxicology 8:813–823
Gopinath SCB, Lakshmipriya T, Awazu K (2014) Colorimetric detection of controlled assembly and disassembly of aptamers on unmodified gold nanoparticles. Biosens Bioelectron 51:115–123
Haes AJ, Duyne RPV (2004) Preliminary studies and potential applications of localized surface plasmon resonance spectroscopy in medical diagnostics. Expert Rev Mol Diagn 4:527–537
Han MS, Lytton-Jean AKR, Oh B-K, Heo J, Mirkin CA (2006) Colorimetric screening of dna-binding molecules with gold nanoparticle probes angewandte. Angew Chem Int Edit 45:1807–1810
Hermanson GT (2008) Bioconjugate techniques. 2nd edn, Academic Press, San Deigo, pp 595–596
Kesik M, Kanik FE, Hizalan G, Kozanoglu D, Esenturk EN, Timur S, Toppare L (2013) A functional immobilization matrix based on a conducting polymer and functionalized gold nanoparticles: synthesis and its application as an amperometric glucose biosensor. Polymer 54:4463–4471
Liu G, Guo W, Yin Z (2014) Covalent fabrication of methyl parathion hydrolase on gold nanoparticles modified carbon substrates for designing a methyl parathion biosensor. Biosens Bioelectron 53:440–446
Louis C, Bond G, Pluchery O (2012) Gold nanoparticles for physics, chemistry and biology. Imperial College Press, London, UK, pp 355–373
Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382:607–609
Parak WJ, Gerion D, Zanchet D et al (2002) Conjugation of DNA to Silanized colloidal semiconductor nanocrystalline quantum dots. Chem Mater 14:2113–2119
Schreiner SM, Hatch AL, Shudy DF et al (2011) Impact of DNA-surface interactions on the stability of dna hybrids. Anal Chem 83:4288–4295
Smith BD, Dave N, Huang P-JJ, Liu J (2011) Assembly of DNA-functionalized gold nanoparticles with gaps and overhangs in linker DNA. J Phys Chem C 115:7851–7857
Storhoff JJ, Lazarides AA, Mucic RC, Mirkin CA, Letsinger RL, Schatz GC (2000) What controls the optical properties of dna-linked gold nanoparticle assemblies? J Am Chem Soc 122:4640–4650
Tan D, He Y, Xing X, Zhao Y, Tang H, Pang D (2013) Aptamer functionalized gold nanoparticles based fluorescent probe for the detection of mercury (II) ion in aqueous solution. Talanta 113:26–30
Zanchet D, Micheel CM, Parak WJ, Gerion D, Alivisatos AP (2000) Electrophoretic isolation of discrete Au nanocrystal/DNA conjugates. Nano Lett 1:32–35
Supporting information
Supplementary Table 1—Sequences of oligonucleotides characterization of modified GNPs.
Supplementary Fig. 1—Agarose gel electrophoresis of GNPs with dsDNA.
Supplementary Fig. 2—Limits of detection preparation of GNPs.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Xing, M., Li, F., Dong, Y. et al. A method to label biological molecules with dsDNA coated gold nanoparticles. Biotechnol Lett 37, 545–549 (2015). https://doi.org/10.1007/s10529-014-1722-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-014-1722-z