Abstract
Oxidative stress is associated with metabolite formation in fungi. In contrast to an Aspergillus niger wild-type strain, a sclerotia-formation regulator ansclR deletion strain demonstrated increased susceptibility to oxidative stress and reduced transcription of the catalase gene, catB, while an ansclR overexpression strain showed enhanced resistance to oxidative stress and increased expression of catB. In addition, ansclR complementation strain expressed a wild-type level of catB. The ansclR overexpression strain also produced the same metabolites as the wild type strain treated with H2O2. Furthermore, LC–MS, NMR, and IR analyses showed that the main metabolite was a steroid analog. Our study adds new clues to oxidative stress-related factors and metabolite formation in A. niger.
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Acknowledgments
This work was supported by the 863 (Hi-tech research and development program of China) program (No. 2012AA022108) and by the Guangdong Provincial Department of Science and Technology Research Project (Nos. 2012B010900028 and 2012A080800013).
Supporting information
Supplementary Table 1 – Strains and plasmids used in this study.
Supplementary Table 2 – Primers used in this study.
Supplementary Figure 1 – Construction of the ansclR deletion, complementation and ansclR overexpression strains.
Supplementary Figure 2 – Antibacterial activity analysis.
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Lv, Y., Zhou, F., Wang, B. et al. Morphological transitions under oxidative stress in response to metabolite formation in Aspergillus niger . Biotechnol Lett 37, 601–608 (2015). https://doi.org/10.1007/s10529-014-1713-0
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DOI: https://doi.org/10.1007/s10529-014-1713-0