Abstract
Lentivirus vectors encoding Wnt10b gene (LV–Wnt10b) or luciferase gene (LV-luc) were constructed to determine whether Wnt10b overexpression improved fracture healing in a rat atrophic non-union model. After fracture, rats were injected with LV-Wnt10b or LV-luc. Luciferase signals were clearly detected. At 2 and 4 weeks, LV-Wnt10b group had 107 and 98 % more proliferating cell nuclear antigen (PCNA) positive cells, respectively, and promoted expression of bone morphogenetic protein-2 (BMP-2) in the callus compared with controls. LV-Wnt10b injection significantly increased bone mass density and bone mineral content: 46–84 and 96–193 %, respectively, at the site of fracturein the LV-Wnt10b group compared with controls. At 8 weeks, fractured femora were healed in the LV-Wnt10b group compared with atrophic non-unions formed in controls. Thus, Wnt10b overexpression associated with lentiviral gene therapy is effective in healing atrophic non-unions in rats.
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Supporting information
Supplementary Figure 1 – HE staining showing bone formation and unions at the fracture site in the LV-luc (a) and LV-Wnbt10 group (b) at 8 weeks post-fracture.
Supplementary Figure 2 – Radiographs of atrophic non-union models in the LV-luc (a, c, e, g) and LV-Wnt10b group (b, d, f, h) obtained at 2 weeks (a, b), 4 weeks (c, d), 6 weeks (e, f) and 8 weeks after surgery (g, h).
Supplementary Figure 3 – LV-Wnt10b treatment enhances total bone mineral density (BMD) (A) and bone mineral content (BMC) (B) in the fracture callus.
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Gao, F., Zhang, Cq., Chai, Ym. et al. Lentivirus-mediated Wnt10b overexpression enhances fracture healing in a rat atrophic non-union model. Biotechnol Lett 37, 733–739 (2015). https://doi.org/10.1007/s10529-014-1703-2
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DOI: https://doi.org/10.1007/s10529-014-1703-2