Abstract
Acetone/butanol/ethanol (ABE) fermentation by Clostridium acetobutylicum was investigated in extractive fed-batch experiments. In conventional fermentations, metabolic activity ceases when a critical threshold products concentration is reached (~21.6 g solvents l−1). Solvents production was increased up to 36.6 and 37.2 g l−1, respectively, using 2-butyl-1-octanol (aqueous to organic ratio: 1:0.25 v/v) and pomace olive oil (1:1 v/v) as extraction solvents. The morphological changes of different cell types were monitored and quantified using flow cytometry. Butanol production in extractive fermentations with pomace olive oil was achieved mainly by vegetative cells, whereas the percentage of sporulating cells was lower than 10 %.
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Acknowledgments
We would like to thank IFP Energies Nouvelles for their financial support, and for their technical and scientific advice. The authors (TALC, MTM and JML) belong to the Galician Competitive Research Group GRC 2013-032.
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González-Peñas, H., Lu-Chau, T.A., Moreira, M.T. et al. Assessment of morphological changes of Clostridium acetobutylicum by flow cytometry during acetone/butanol/ethanol extractive fermentation. Biotechnol Lett 37, 577–584 (2015). https://doi.org/10.1007/s10529-014-1702-3
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DOI: https://doi.org/10.1007/s10529-014-1702-3