Skip to main content
Log in

Use of a whole-cell bioreporter, Acinetobacter baylyi, to estimate the genotoxicity and bioavailability of chromium(VI)-contaminated soils

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

An Erratum to this article was published on 01 March 2015

Abstract

A whole-cell bioreporter, Acinetobacter baylyi ADPWH_recA, was used to estimate the genotoxicity and bioavailability of chromium (VI) [Cr(VI)] in contaminated soils. Upon direct exposure to pre-sonicated soil samples, ADPWH_recA gave the highest response to the genotoxicity of Cr(VI) within 5 h with a detection limit of 2 µM Cr(VI). Investigations on sites contaminated with Cr(VI) revealed that soil-associated Cr(VI) was bioavailable to the bioreporter although it could not be extracted into the aqueous phase. The physical and chemical properties of soil might influence the bioavailability of Cr(VI), and higher genotoxicity was found in soils with a lower pH. This whole cell bioreporter approach makes it feasible to evaluate the bioavailability and genotoxicity of Cr(VI)-contaminated soils to uncover their potential impact on human health.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abollino O, Aceto M, Malandrino M, Sarzanini C, Mentasti E (2003) Adsorption of heavy metals on Na-montmorillonite. Effect of pH and organic substances. Water Res 37:1619–1627

    Article  CAS  PubMed  Google Scholar 

  • Ackerley DF, Barak Y, Lynch SV, Curtin J, Matin A (2006) Effect of chromate stress on Escherichia coli K-12. J Bacteriol 188:3371–3381

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Anderson RA, Bryden NA, Polansky MM (1997) Lack of toxicity of chromium chloride and chromium picolinate in rats. J Am Coll Nutr 16:273–279

    Article  CAS  PubMed  Google Scholar 

  • Bao SQ (2008) Soil agrochemical analysis, 3rd edn. Agricultural Publishing House of China, Beijing

    Google Scholar 

  • Huang WE, Wang H, Zheng HJ, Huang LF, Singer AC, Thompson I, Whiteley AS (2005) Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the detection of salicylate. Environ Microbiol 7:1339–1348

    Article  CAS  PubMed  Google Scholar 

  • Ivask A, François M, Kahru A, Dubourguier HC, Virta M, Douay F (2004) Recombinant luminescent bacterial sensors for the measurement of bioavailability of cadmium and lead in soils polluted by metal smelters. Chemosphere 55:147–156

    Article  CAS  PubMed  Google Scholar 

  • Liao VHC, Chien MT, Tseng YY, Ou KL (2006) Assessment of heavy metal bioavailability in contaminated sediments and soils using green fluorescent protein-based bacterial biosensors. Environ Pollut 142:17–23

    Article  CAS  PubMed  Google Scholar 

  • Llagostera M, Garrido S, Guerrero R, Barbé J (1986) Induction of SOS genes of Escherichia coli by chromium compounds. Environ Mutagen 8:571–577

    Article  CAS  PubMed  Google Scholar 

  • Marqués MJ, Salvador A, Morales-Rubio A, de la Guardia M (2000) Chromium speciation in liquid matrices: a survey of the literature. Fresenius J Anal Chem 367:601–613

    Article  PubMed  Google Scholar 

  • Petrilli FL, Flora SD (1977) Toxicity and mutagenicity of hexavalent chromium on Salmonella typhimurium. Appl Environ Microbiol 33:805–809

    PubMed Central  CAS  PubMed  Google Scholar 

  • Song Y et al (2014) A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China. Environ Pollut. doi:10.1016/j.envpol.2014.08.024

  • Song YZ, Li GH, Thornton SF, Thompson LP, Banwart SA, Lerner DN, Huang WE (2009) Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples. Environ Sci Technol 43:7931–7938

    Article  CAS  PubMed  Google Scholar 

  • van der Meer JR, Belkin S (2010) Where microbiology meets microengineering: design and applications of reporter bacteria. Nat Rev Microbiol 8:511–522

    Article  PubMed  Google Scholar 

  • Wazne M, Moon DH, Jagupilla SC, Jagupilla SC, Christodoulatos C, Dermatas D, Chrysochoou M (2007) Remediation of chromite ore processing residue using ferrous sulfate and calcium polysulfide. Geosci J 11:105–110

    Article  CAS  Google Scholar 

  • Zhang DY, Fakhrullin RF, Özmen M, Wang H, Wang J, Paunov VN, Li GH, Huang WE (2011) Functionalization of whole-cell bacterial reporters with magnetic nanoparticles. Microb Biotechnol 4:89–97

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang DY, He Y, Wang Y, Wang H, Wu L, Aries E, Huang WE (2012) Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microb Biotechnol 5:87–97

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank the Chinese Scholarship Council for providing a studentship to B.J. This work was supported by the Tsinghua University Initiative Scientific Research Program (20121080049), Ministry of Science and Technology of China (2012FY130300) and National Natural Science Foundation of China (41301331).

Supporting information

Supplementary Figure 1—Kinetic bioluminescent intensity of ADPWH_recA induced by artificial Cr-contaminated soils. The soils were all treated with ultrasound for 300 s and exposed to the bioreporter as soil-water slurries. Three replicates were conducted for each soil sample and three independent replicates were carried out for the whole experiment. Error bars were standard deviations.

Supplementary Figure 2—Cell viability estimation of ADPWH_recA exposed to different concentrations of Cr(VI). The 100 μM, 10 μM and 1 μM samples were artificial Cr(VI)-contaminated soils and S7 was the Cr(VI)-contaminated soils taken from Shandong Province. 200 mg of these soils were mixed with 5 ml Milli-Q water and sonicated for 300 s as water-soil slurries. The cell population estimating was done by colony couting on LB agar plate. Bioreporter cells exposed to different concentrations of Cr(VI) were sampled at 0 h, 3 h, 4 h, 5 h and 6 h, and made a series dilution of 101, 102, 103, 104, 105 and 106. The 100 μl of each diluted cells were spread on LB agar plate and incubated at 30 °C for colony counting. Three replicates were carried out for each treatment and three independent replicates were carried out for the whole experiment. Error bars were standard deviations.

Supplementary Figure 3—Sample collection from chromium-contaminated sites in China.

Supplementary Table 1—Physical and chemical properties of chromium-contaminated soil samples.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Guanghe Li.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 431 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiang, B., Zhu, D., Song, Y. et al. Use of a whole-cell bioreporter, Acinetobacter baylyi, to estimate the genotoxicity and bioavailability of chromium(VI)-contaminated soils. Biotechnol Lett 37, 343–348 (2015). https://doi.org/10.1007/s10529-014-1674-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-014-1674-3

Keywords

Navigation