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Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy

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Abstract

Among various enzyme-based therapies, enzyme-prodrug therapy (EPT) promises minimized side effects in that it activates non-toxic prodrugs locally where the enzymes are placed. The success of such an approach requires high enzyme stability against both structural denaturation and potential immunogenicity. This work examines the efficiency of nanoparticles for enzyme protection in EPT applications. Specifically, horseradish peroxidase (HRP)-encapsulated chitosan nanoparticles (HRP-CSNP) were constructed and examined with respect to stability enhancement. HRP-CSNP retained enzyme activity and had improved stability at 37 °C in the presence of a denaturant, urea. The nanoparticles effectively bound to the surface of human breast cancer cell Bcap37 and led to over 80 % cell death when applied with a prodrug indole-3-acetic acid.

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Acknowledgments

This work was supported by seed grant for cultivating and interdisciplinary research of Chinese Education Ministry, National Natural Science Foundation of China (21303050), China Postdoctoral Science Foundation Grant (2013M540341), and National “Thousand Talents Program” of China.

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Authors and Affiliations

  1. Biomedical Nanotechnology Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Xiaodan Cao, Chao Chen, Haijun Yu & Ping Wang

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  1. Xiaodan Cao
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  2. Chao Chen
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  3. Haijun Yu
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  4. Ping Wang
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Correspondence to Ping Wang.

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Cao, X., Chen, C., Yu, H. et al. Horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy. Biotechnol Lett 37, 81–88 (2015). https://doi.org/10.1007/s10529-014-1664-5

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  • DOI: https://doi.org/10.1007/s10529-014-1664-5

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