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Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress

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Abstract

Improper fluid shear stress (FSS) can cause serious damages to bone marrow mesenchymal stem cells (MSCs). Mechano-growth factor (MGF) E peptide pretreatment was proposed to protect MSCs against FSS damage in this study. MSCs were exposed to FSS for 30 min after they were pretreated with MGF E peptide for 24 h. Then, the effects of MGF E peptide on the viability, proliferation and cell apoptosis of MSCs were investigated. MGF E peptide pretreatment could recover the cellular metabolic activity of MSCs reduced by 72 dyne cm−2 FSS and had a synergistic effect with FSS on the cellular metabolic viability of MSCs under 24 and 72 dyne cm−2 FSS. These results suggested that MGF E peptide pretreatment could be an effective method for the protection of FSS damage in bone tissue engineering.

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Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (11172338, 11032012), the Program for New Century Excellent Talents in University (NCET-10-0879), the Fundamental Research Funds for the Central Universities (CDJZR 12238801, CQDXWL-2012-Z001), and the Sharing Fund of Chongqing University’s Large-scale Equipment.

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Authors and Affiliations

  1. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, P. R., China

    Yonggang Lv, Xiaoying Hao, Yongqiang Sha & Li Yang

  2. ‘111’ Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, P. R., China

    Yonggang Lv, Xiaoying Hao, Yongqiang Sha & Li Yang

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  1. Yonggang Lv
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Correspondence to Yonggang Lv.

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Lv, Y., Hao, X., Sha, Y. et al. Pretreatment with mechano-growth factor E peptide protects bone marrow mesenchymal cells against damage by fluid shear stress. Biotechnol Lett 36, 2559–2569 (2014). https://doi.org/10.1007/s10529-014-1625-z

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  • DOI: https://doi.org/10.1007/s10529-014-1625-z

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