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Characterization of industrially-valuable xylano-pectinolytic enzymes produced concurrently by a novel isolate of Bacillus pumilus

Abstract

Xylanase and polygalacturonase were concurrently produced by a novel alkalo-thermotolerant Bacillus pumilus AJK. They were purified and characterized to evaluate their potential for various industrial applications. Xylanase was purified to 19-fold with 67 % recovery and polygalacturonase up to 23-fold with 75 % recovery. Existence of multiple forms of xylanase was indicated by its elution-profile through Sephadex G-100 as two peaks, xylanase-I and xylanase-II, with molecular weights of ~24.5 and ~13 kDa, respectively, and by the presence of two pH optima, one at pH 6.0 and other at pH 8.5. The molecular weight of polygalacturonase was ~40 kDa by gel-filtration chromatography. Zymographic studies confirmed the presence of seven isozymic forms of xylanase. Xylanase and polygalacturonase are stable over a broad range of pH and temperature.

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Acknowledgments

The authors acknowledge the financial support provided by University Grant Commission, India. University Research Scholarship awarded to Amanjot Kaur by Kurukshetra University is also kindly acknowledged.

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The authors declare that they have no conflict of interest.

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Correspondence to Ritu Mahajan.

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Kaur, A., Singh, A. & Mahajan, R. Characterization of industrially-valuable xylano-pectinolytic enzymes produced concurrently by a novel isolate of Bacillus pumilus . Biotechnol Lett 36, 2229–2237 (2014). https://doi.org/10.1007/s10529-014-1595-1

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Keywords

  • Bacillus pumilus
  • Pectinase
  • Polygalacturonase
  • Purification
  • Ultrafiltration
  • Xylanase
  • Xylano-pectinolytic enzyme
  • Zymography